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公开(公告)号：US20250137002A1
公开(公告)日：2025-05-01
申请号：US18983993
申请日：2024-12-17
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , A01H4/00 , A01H6/20 , A01H6/46 , A01H6/54 , A01H6/60 , C12N1/20 , C12N5/00 , C12N5/04 , C12N9/10 , C12R1/41 , G01N33/00
Abstract: The present invention relates to methods and compositions for transforming soybean, corn. cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US11542514B2
公开(公告)日：2023-01-03
申请号：US17124115
申请日：2020-12-16
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
IPC: C12N15/82 , C12N5/00 , A01H4/00 , C12N5/04 , G01N33/00 , C12N9/10 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60 , C12N1/20 , C12R1/41
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20210332375A1
公开(公告)日：2021-10-28
申请号：US17317255
申请日：2021-05-11
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, JR. , Brian J. Martinell , Jyoti R. Rout , Edward J. Williams
IPC: C12N15/82
Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
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公开(公告)号：US10918032B2
公开(公告)日：2021-02-16
申请号：US16106342
申请日：2018-08-21
Applicant: Monsanto Technology LLC
Inventor： Whitney R. Adams, Jr. , Brandon Davis , Lubomyr Kucher , Brian J. Martinell , Jyoti R. Rout , Brenda A. Lowe
Abstract: The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.
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公开(公告)号：US20150184170A1
公开(公告)日：2015-07-02
申请号：US14642671
申请日：2015-03-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
Abstract translation: 本发明涉及从植物组织培养和遗传转化中切除包含来自种子的分生组织的外植体材料，以及这些材料的储存。 公开了组织制备，储存和转化的方法，以及通过这些方法生产的可转化的分生组织，以及组织制备。
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Patent Agency Ranking
公开(公告)号：US09006513B2
公开(公告)日：2015-04-14
申请号：US13750977
申请日：2013-01-25
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
Abstract translation: 本发明涉及从植物组织培养和遗传转化中切除包含来自种子的分生组织的外植体材料，以及这些材料的储存。 公开了组织制备，储存和转化的方法，以及通过这些方法生产的可转化的分生组织，以及组织制备。
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公开(公告)号：US08872000B2
公开(公告)日：2014-10-28
申请号：US13899452
申请日：2013-05-21
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
Abstract translation: 本发明涉及使用壮观霉素作为转化外植体的选择剂转化大豆，玉米，棉花或油菜外植体的方法和组合物。 该方法还可以包括在转化和再生过程中用细胞分裂素处理外植体。
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公开(公告)号：US20240301435A1
公开(公告)日：2024-09-12
申请号：US18609293
申请日：2024-03-19
Applicant: Monsanto Technology LLC
Inventor： Justin Arsenault , Yurong Chen , Byung-Guk Kang , Jennifer Kumpf , Brian J. Martinell , Lorena B. Moeller , Mary Ann Saltarikos , Ashok Shrawat , Shubha Subbarao , Edward J. Williams , Xudong Ye
CPC classification number: C12N15/8205 , A01H4/008
Abstract: The invention provides novel compositions and methods for improving the transformation of monocot seed excised embryo explants, which may include one or more steps of explant preparation, explant rehydration, Rhizobiales bacterium inoculation and co-culture, bud induction, extended bud induction, or regeneration of genetically modified plants or plant parts. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide by inoculating the embryo explant with a Rhizobiales bacterium comprising the heterologous polynucleotide. The methods provided herein also include methods of regenerating a genetically modified plant or plant part from a transformed or edited plant cell or explant.
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公开(公告)号：US20240117366A1
公开(公告)日：2024-04-11
申请号：US18481464
申请日：2023-10-05
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC classification number: C12N15/8209 , A01H4/003 , A01H4/008 , C12N5/0025 , C12N5/04 , C12N9/1029 , C12N15/8201 , C12N15/8202 , C12N15/8205 , C12N15/8221 , C12N15/8265 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , G01N33/0098 , A01H6/202 , C12N15/8261
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US11485980B2
公开(公告)日：2022-11-01
申请号：US16904347
申请日：2020-06-17
Applicant: Monsanto Technology LLC
Inventor： Erik Dersch , Richard J. Heinzen , Brian J. Martinell , Anatoly Rivlin , Yuechun Wan , Xudong Ye
IPC: C12N15/82 , C12N5/00 , A01H4/00 , C12N5/04 , G01N33/00 , C12N9/10 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60 , C12N1/20 , C12R1/41
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from cotton seeds. Methods for tissue preparation, storage, transformation, and selection or identification of transformed plants are disclosed, as are transformable meristem tissues and plants produced by such methods, and apparati for tissue preparation.
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