公开(公告)号：US20140080755A1

公开(公告)日：2014-03-20

申请号：US13960646

申请日：2013-08-06

Applicant: Monsanto Technology LLC

Inventor： Gregory R. Heck ,  Tichafa R.I. Munyikwa ,  Jean C. Goley ,  James K. Roberts ,  Scott C. Johnson ,  Ty T. Vaughn

IPC: C12N15/113

CPC classification number: C12N15/113 ,  C12N15/111 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  C12N2320/10 ,  Y02A40/162

Abstract: The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.

Abstract translation: 本发明提供了选择在目标生物体中产生dsRNA介导的基因抑制并使其能够被靶生物吸收的核苷酸序列的方法。 本发明进一步提供赋予转基因宿主细胞中此类序列稳定表达的表达构建体及其使用方法。 还提供了通过本发明的方法制备的生物，细胞和组织。

公开(公告)号：US11708577B2

公开(公告)日：2023-07-25

申请号：US16701782

申请日：2019-12-03

Applicant: Monsanto Technology LLC

Inventor： Joanne Fillatti ,  Jean C. Goley ,  Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

公开(公告)号：US20180002712A1

公开(公告)日：2018-01-04

申请号：US15702384

申请日：2017-09-12

Applicant: Monsanto Technology LLC

Inventor： Joanne Fillatti ,  Jean C. Goley ,  Gregory R. Heck ,  Tichafa R.I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

公开(公告)号：US09765351B2

公开(公告)日：2017-09-19

申请号：US14322393

申请日：2014-07-02

Applicant: Monsanto Technology LLC

Inventor： Joanne Fillatti ,  Jean C. Goley ,  Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.