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    Invertebrate microRNAs
    2.
    发明授权
    Invertebrate microRNAs 有权
    无脊椎动物微RNA

    公开(公告)号：US09528121B2

    公开(公告)日：2016-12-27

    申请号：US13746500

    申请日：2013-01-22

    Applicant: Monsanto Technology LLC

    Inventor： Edwards Allen ,  William P. Donovan ,  Gregory R. Heck ,  James K. Roberts ,  Virginia Ursin ,  Yuanji Zhang

    IPC: A01H1/00 ,  C12N15/82 ,  C12N15/87 ,  A01N63/02 ,  C12N15/113

    CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/113 ,  C12N15/8285 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/531 ,  Y02A40/162 ,  Y02A40/164

    Abstract: This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

    Abstract translation: 本发明提供具有抗无脊椎害虫的植物。 更具体地，本发明公开了一种非天然转基因植物细胞，其在植物中表达至少一种无脊椎动物miRNA，用于抑制无脊椎害虫或与无脊椎害虫相关的共生体的靶基因。 还提供了用于在植物中表达至少一种无脊椎动物miRNA的重组DNA构建体，包含本发明的非天然转基因植物细胞的非天然转基因植物，从非天然转基因植物细胞生长的非天然转基因植物 以及由非天然转基因植物产生的非天然转基因种子，以及由本发明的非天然转基因植物细胞，植物或种子产生的商品。 本发明还提供一种抑制植物无脊椎动物害虫或与无脊椎动物相关共生体的至少一种靶基因的方法，包括提供包含本发明非天然转基因植物细胞的植物，其中无脊椎动物是 无脊椎动物害虫，重组DNA在非天然转基因植物细胞中转录至重组miRNA前体，当无脊椎害虫摄入重组miRNA前体时，抑制至少一种靶基因。

    Modified Gene Silencing
    6.
    发明申请
    Modified Gene Silencing 审中-公开

    公开(公告)号：US20180002712A1

    公开(公告)日：2018-01-04

    申请号：US15702384

    申请日：2017-09-12

    Applicant: Monsanto Technology LLC

    Inventor： Joanne Fillatti ,  Jean C. Goley ,  Gregory R. Heck ,  Tichafa R.I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

    Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

    Modified gene silencing
    8.
    发明授权
    Modified gene silencing 有权

    公开(公告)号：US09765351B2

    公开(公告)日：2017-09-19

    申请号：US14322393

    申请日：2014-07-02

    Applicant: Monsanto Technology LLC

    Inventor： Joanne Fillatti ,  Jean C. Goley ,  Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

    Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

    MODIFIED GENE SILENCING
    10.
    发明申请
    MODIFIED GENE SILENCING 有权
    改良基因沉默

    公开(公告)号：US20140315312A1

    公开(公告)日：2014-10-23

    申请号：US14322393

    申请日：2014-07-02

    Applicant: Monsanto Technology LLC

    Inventor： Joanne Fillatti ,  Jean C. Coley ,  Gregory R. Heck ,  Tichafa R. I. Munyikwa ,  Ty T. Vaughn ,  Toni Voelker

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8218 ,  C12N15/8247

    Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

    Abstract translation: 本发明涉及控制真核细胞中基因表达或基因抑制的方法。 本发明的一个方面包括通过使用修饰的抑制元件来修饰靶基因的沉默程度。 另一方面包括提供具有由修饰的抑制元件的真核细胞中转录产生的所需表型的真核细胞。 还提供了转基因真核细胞，转基因植物细胞，植物和含有修饰的抑制元件的种子，以及这种转基因植物细胞，植物或种子如食物或饲料产品的有用衍生物。

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