公开(公告)号：US4591571A

公开(公告)日：1986-05-27

申请号：US601522

申请日：1984-04-18

申请人： Morio Kuboyama ,  Yoshitsugu Harada ,  Akio Kawashiri ,  Eiji Takahashi

发明人： Morio Kuboyama ,  Yoshitsugu Harada ,  Akio Kawashiri ,  Eiji Takahashi

IPC分类号： A61K39/44 ,  G01N33/531 ,  G01N33/543 ,  G01N33/546

CPC分类号： G01N33/54393 ,  G01N33/531 ,  Y10S436/825

摘要： Reagents for detecting antigen contained in body fluid or urine according to the immunological agglutination or agglutination inhibition reaction and comprising an antibody adsorbed on fine carrier particle to be sensitized as an effective constituent, and is characterized by acylating said antibody.Owing to such acylation, the reagent can correctly detect antigen without any influence of substance contained in sample which has inevitably caused nonspecific agglutination reaction according to the prior art.

摘要翻译： 根据免疫凝集或凝集抑制反应检测体液或尿中含有的抗原的试剂，其含有被吸附在敏化剂作为有效成分的细载体颗粒上的抗体，其特征在于酰化所述抗体。 由于这样的酰化，试剂可以正确检测抗原，而不受样品中所含物质的任何影响，根据现有技术不可避免地引起非特异性凝集反应。

公开(公告)号：US4226847A

公开(公告)日：1980-10-07

申请号：US917254

申请日：1978-06-20

申请人： Katsuhiro Ogasa ,  Morio Kuboyama ,  Minoru Saito ,  Tsutomu Kudo ,  Yoshitsugu Harada ,  Akio Kawashiri ,  Eiji Takahashi

发明人： Katsuhiro Ogasa ,  Morio Kuboyama ,  Minoru Saito ,  Tsutomu Kudo ,  Yoshitsugu Harada ,  Akio Kawashiri ,  Eiji Takahashi

IPC分类号： G01N33/53 ,  A61B10/00 ,  G01N33/15 ,  G01N33/531 ,  G01N33/543 ,  A61K39/00 ,  A61K31/74 ,  G01N31/00 ,  G01N33/16

CPC分类号： G01N33/54313 ,  Y10S436/817 ,  Y10S530/815

摘要： Latex reagents for quantitative assay of a variety of steroid hormones or metabolites thereof which are contained in body fluid or excreted fluid of human beings comprise latex particles which are immunologically sensitized with a conjugate selected from a variety of steroid-serum albumin conjugates in which the steroid to be detected is bonded to serum albumin in a very small ratio ranging from 0.5 to 7 molecules per 1 molecule of the serum albumin used.Sensitization of latex particles with such a conjugate is performed using a limited amount dependent on the bonding ratio of steroid molecules per molecule of serum albumin in the conjugate and the particle size of the latex used.The latex reagents show very high sensitivity amounting to 0.2-0.04 nmole steroid equivalent/ml.

摘要翻译： 用于定量测定体液或人体排泄液中各种类固醇激素或其代谢物的胶乳试剂包含用选自多种类固醇 - 血清白蛋白缀合物的缀合物进行免疫敏化的胶乳颗粒，其中类固醇 要被检测的是以每1分子所用血清白蛋白0.5至7个分子的非常小的比例与血清白蛋白结合。 使用这样的缀合物对胶乳颗粒进行敏化，其取决于缀合物中每分子血清白蛋白的类固醇分子的键合比例和所使用的胶乳的粒径。 乳胶试剂显示非常高的灵敏度，达到0.2-0.04nmol类固醇当量/ ml。