专利检索 ap:("Motohiro Otsuka" OR "Hiroshi Mizuno" OR "Frederic Tsuji" OR "Hiromi Takenaka") AND inv:"Frederic Tsuji" 第 1 页

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发明申请
Method of isolating and purifying aequorin, aequorin produced by the method, and process for detecting calcium ions with aequorin 审中-公开
标题翻译：通过该方法分离和纯化水母发光蛋白，水母发光蛋白的方法和用水母发光蛋白检测钙离子的方法

公开(公告)号：US20050054838A1

公开(公告)日：2005-03-10

申请号：US10895900

申请日：2004-07-22

申请人： Motohiro Otsuka , Hiroshi Mizuno , Frederic Tsuji , Hiromi Takenaka

发明人： Motohiro Otsuka , Hiroshi Mizuno , Frederic Tsuji , Hiromi Takenaka

IPC分类号： G01N33/50 , B01D15/08 , C07K14/435 , C12N9/02 , C12Q1/26 , G01N21/78 , G01N30/34 , G01N30/88 , C12Q1/68 , A23J1/00 , C07K1/00 , C07K14/00 , C07K16/00 , C07K17/00

CPC分类号： C12N9/0069 , C07K14/43595

摘要： Isoforms of apoaequorin and isoforms of aequorin are isolated and purified from recombinant apoaequorin and a solution containing regenerated aequorin, respectively, by gradient elution chromatography. As a result, aequorin can be isolated and purified.

摘要翻译： 通过梯度洗脱色谱分别从重组载脂蛋白和含有再生的水母发光蛋白的溶液中分离和纯化脱辅基水母蛋白的同工型和水母发光蛋白的同种型。结果，可以分离和纯化水母发光蛋白。