公开(公告)号：US20150021496A1

公开(公告)日：2015-01-22

申请号：US13944043

申请日：2013-07-17

Applicant: Muhammad Imran Shabbir

Inventor： Muhammad Imran Shabbir

IPC: G01N27/447 ,  G01B5/25 ,  G01N21/64

CPC classification number: G01N27/44721 ,  G01N21/64 ,  G01N27/44726 ,  G01N2021/6495 ,  G01N2021/6497

Abstract: A method to assist gel analysis and processing and apparatus for the same is disclosed. The method employs a tracing/marking/measuring/analyzing and referencing template of transparent sheet material comprising of a pattern made of vertical and horizontal lines that form quadrilateral shapes upon intersection. The vertical and horizontal lines are clearly visible in white light and ultraviolet light. The template sheet(s) can be inserted in a square shaped template holding assembly (70) which is used in between a UV trans-illuminator (56) and a macromolecule resolving gel matrix (54) such as agarose or polyacrylamide gels. The template pattern can be made on one sheet of transparent material or separate sheets may contain vertical and horizontal lines and the finalized pattern can be achieved by inserting all the separate sheets in the template holding assembly. Under the UV light from UV trans-illuminator by adjusting the sheets containing vertical lines (13) according to the gel matrix's lanes (32d, 32e, 32f) and horizontal lines (17) according to the area of interest for further analysis and processing, the adjusted pattern of lines (13) and (17) can be used as a reference even in the absence of UV light.

Abstract translation: 公开了一种辅助凝胶分析和处理的方法及其装置。 该方法采用透明片材的跟踪/标记/测量/分析和参考模板，其包括由交叉点形成四边形形状的垂直和水平线形成的图案。 垂直和水平线在白光和紫外线下清晰可见。 模板片可以插入在UV反射照明器（56）和大分子拆分凝胶基质（54）如琼脂糖或聚丙烯酰胺凝胶之间的方形模板保持组件（70）中。 模板图案可以在一片透明材料上制成，或者单独的片材可以包含垂直和水平线，并且可以通过将所有分离的片材插入模板保持组件中来实现最终化图案。 根据感兴趣的区域，通过根据凝胶基质通道（32d，32e，32f）和水平线（17）调整含有垂直线（13）的片材在UV反射照明器的UV光下进行进一步分析和处理， 即使在没有UV光的情况下，也可以将线（13）和（17）的调整图案用作基准。