公开(公告)号：US20130316376A1

公开(公告)日：2013-11-28

申请号：US13880628

申请日：2011-10-25

申请人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

发明人： My Hedhammar ,  Jan Johansson ,  Anna Rising ,  Per Åke Nygren

IPC分类号： C07K14/435

CPC分类号： C07K14/43518 ,  B01D15/20 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3823 ,  B01J20/281 ,  C07D495/04 ,  C07K14/31 ,  C07K14/76 ,  C07K16/065 ,  C07K2319/01 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/70 ,  C07K2319/705 ,  C12N5/0068 ,  C12N2533/50 ,  Y10S210/905

摘要： A protein structure capable of selective interaction with an organic target is provided. The protein structure is a polymer comprising as a repeating structural unit a recombinant fusion protein that is capable of selective interaction with the organic target. The fusion protein is comprising the moieties B, REP and CT, and optionally NT. B is a non-spidroin moiety of more than 30 amino acid residues, which provides the capacity of selective interaction with the organic target. REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein. NT is an optional moiety of from 100 to 160 amino acid residues and is derived from the N-terminal fragment of a spider silk protein. The fusion protein and protein structure thereof is useful as an affinity medium and a cell scaffold material.

公开(公告)号：US06267964B1

公开(公告)日：2001-07-31

申请号：US08162906

申请日：1993-12-08

申请人： Per Åke Nygren ,  Hans Wigzell ,  Mathias Uhlén

发明人： Per Åke Nygren ,  Hans Wigzell ,  Mathias Uhlén

IPC分类号： A61K3900

CPC分类号： C07K14/315 ,  A61K38/00 ,  A61K47/646 ,  C07K14/31 ,  C07K2319/00

摘要： A process for extending the half-life in vivo by a biologically active protein or peptide, characterized by covalently coupling said protein or peptide to a polypeptide fragment capable of binding to a serum protein, whereby when administering the resulting protein or peptide conjugate its binding to the serum protein results in extended biological activity, the use of the protein or peptide conjugate above for manufacturing a medicament which, when administered to a mammal including man, shows extended half-life in vivo; and a method of therapeutic or prophylactic treatment.

摘要翻译： 一种通过生物活性蛋白质或肽在体内延长半衰期的方法，其特征在于将所述蛋白质或肽共价偶联到能够结合血清蛋白质的多肽片段，由此当施用所得蛋白质或肽缀合物时，其结合 血清蛋白质导致延长的生物学活性，上述蛋白质或肽结合物的使用用于制备当向包括人类的哺乳动物施用时在体内显示延长的半衰期的药物; 以及治疗或预防治疗的方法。