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公开(公告)号：US11104909B2
公开(公告)日：2021-08-31
申请号：US16080277
申请日：2017-02-24
发明人： Yan Fan , Xiuzhen Du , Minghui Hao , Yan Sun , Ke Huang , Feng Li
摘要： Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100° C.
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公开(公告)号：US20180340161A1
公开(公告)日：2018-11-29
申请号：US15989534
申请日：2018-05-25
IPC分类号： C12N9/28
摘要： The present invention discloses an L-type amylase variant and use thereof. The α-amylase variant is obtained by deleting the first N-terminal amino acid residue V from the α-amylase of B. licheniformis and replacing it with three other amino acid residues DGL. The α-amylase variant provided by the present invention has high catalytic activity under the acidic conditions of pH 5.0-5.8 and a high temperature of 100° C. or above. The acid resistance and thermal stability of these α-amylase variants are suitable for starch liquefaction.
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公开(公告)号：US11339397B2
公开(公告)日：2022-05-24
申请号：US16498570
申请日：2018-03-30
发明人： Aixi Bai , Feng Li , Furong Bian , Jidong Zhu , Yan Sun , Hong Xu
摘要： Disclosed herein is a method for expressing phytase in a filamentous fungus by using an optimized Escherichia coli phytase gene having a nucleotide sequence as shown in SEQ ID NO. 7 and a signal peptide having a nucleotide sequence as shown in SEQ ID NO. 12.
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公开(公告)号：US20220220454A1
公开(公告)日：2022-07-14
申请号：US17615342
申请日：2020-05-29
摘要： Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-type Aspergillus niger glucose oxidase or a mutant Aspergillus niger glucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.
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公开(公告)号：US10647970B2
公开(公告)日：2020-05-12
申请号：US15989534
申请日：2018-05-25
摘要： The present invention discloses an L-type amylase variant and use thereof. The α-amylase variant is obtained by deleting the first N-terminal amino acid residue V from the α-amylase of B. licheniformis and replacing it with three other amino acid residues DGL. The α-amylase variant provided by the present invention has high catalytic activity under the acidic conditions of pH 5.0-5.8 and a high temperature of 100° C. or above. The acid resistance and thermal stability of these α-amylase variants are suitable for starch liquefaction.
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公开(公告)号：US20190062764A1
公开(公告)日：2019-02-28
申请号：US16080277
申请日：2017-02-24
发明人： Yan Fan , Xiuzhen Du , Minghui Hao , Yan Sun , Ke Huang , Feng Li
摘要： Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100° C.
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