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公开(公告)号：US20210207112A1
公开(公告)日：2021-07-08
申请号：US17059696
申请日：2019-05-30
IPC分类号： C12N9/16
摘要： Disclosed is a thermostable phytase, in which at least one pair of introduced disulfide bonds is included in the amino acid sequence of wild-type Escherichia coli phytase or mutant Escherichia coli phytase, and after the introduction, the properties of the phytase can be improved, especially the heat stability, steam stability and granulation stability, which are superior to those of the existing wild-type or mutant phytase; compared to the engineered phytase in which disulfide bonds are introduced, the heat stability is also significantly improved.
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公开(公告)号：US11104909B2
公开(公告)日：2021-08-31
申请号：US16080277
申请日：2017-02-24
发明人： Yan Fan , Xiuzhen Du , Minghui Hao , Yan Sun , Ke Huang , Feng Li
摘要： Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100° C.
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公开(公告)号：US10400228B2
公开(公告)日：2019-09-03
申请号：US15113077
申请日：2015-11-10
发明人： Yan Fan , Yamin Lu , Xiuzhen Du , Huadong Du , Feng Li
摘要： Truncated pullulanases having an N-terminal deletion in a parental pullulanase are provided. These truncated pullulanases have altered properties as compared to the parental pullulanase, including an increased saccharification rate and higher catalytic activity at acidic pH values below 4.5 and higher temperatures of up to 64° C. These truncated pullulanase also have improved thermal stability as compared to the parental enzyme. Also provided are compositions containing the truncated pullulanases and a glucoamylase, and processes for applying the truncated pullulanases and compositions in the starch industry. Polynucleotides that encode the truncated pullulanases, and recombinant host cells for producing the truncated pullulanases are also described.
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公开(公告)号：US11339397B2
公开(公告)日：2022-05-24
申请号：US16498570
申请日：2018-03-30
发明人： Aixi Bai , Feng Li , Furong Bian , Jidong Zhu , Yan Sun , Hong Xu
摘要： Disclosed herein is a method for expressing phytase in a filamentous fungus by using an optimized Escherichia coli phytase gene having a nucleotide sequence as shown in SEQ ID NO. 7 and a signal peptide having a nucleotide sequence as shown in SEQ ID NO. 12.
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公开(公告)号：US20190062764A1
公开(公告)日：2019-02-28
申请号：US16080277
申请日：2017-02-24
发明人： Yan Fan , Xiuzhen Du , Minghui Hao , Yan Sun , Ke Huang , Feng Li
摘要： Provided is an α amylase variant obtained by the mutation or deletion of at least one amino acid residue in the amino acid sequence of a parent α amylase, and at the same time, same is an α amylase maintaining the capability of the parent to hydrolyse α-1,4 glycosidic bond, wherein the amino acid sequence homology of the two reaches 95% or more. A series of α amylase variants provided therein have a relatively high catalytic activity under acidic conditions of pH 5.0 and high temperatures of at least 100° C.
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