公开(公告)号：US20230174983A1

公开(公告)日：2023-06-08

申请号：US17921809

申请日：2021-07-13

申请人： NANJING GENEFRIEND-BIOTECH INC.

发明人： Yifan DAI ,  Haiyuan YANG

IPC分类号： C12N15/113 ,  C12N15/85 ,  A01K67/027 ,  C12N9/10 ,  C12N9/22

CPC分类号： C12N15/113 ,  C12N15/85 ,  A01K67/0276 ,  C12N9/1051 ,  C12N9/22 ,  A01K2217/075 ,  A01K2227/108 ,  A01K2267/03 ,  A01K2267/0387 ,  C12N2310/10

摘要： Provided is gRNA specifically targeting β4GalNT2 gene. The gRNA specifically binds to the nucleotide sequence shown in any one of SEQ ID NOs. 1 and 2. Also provided are an animal model constructed using the gRNA, and an application thereof in the field of biomedicine.

公开(公告)号：US20210002652A1

公开(公告)日：2021-01-07

申请号：US16968664

申请日：2019-03-14

申请人： NANJING GENEFRIEND-BIOTECH INC.

发明人： Yifan DAI ,  Haiyuan YANG ,  Ying WANG

IPC分类号： C12N15/66 ,  C12N15/877 ,  C12N15/90 ,  A01K67/027 ,  C12N9/22

摘要： Provided is an SgRNA combination, comprising an SgRNA specifically targeting the GGTA1 gene, an SgRNA specifically targeting the CMAH gene and an SgRNA specifically targeting the β4GalNT2 gene. Also provided is a CRISPR/Cas9 vector combination, comprising a GGTA1-CRISPR/Cas9 vector, a CMAH-CRISPR/Cas9 vector and a β4GalNT2-CRISPR/Cas9 vector. Also provided is an applicaton of the CRISPR/Cas9 vector combination in knocking out the GGTA1 gene, the CMAH gene and the β4GalNT2 gene. The knockout rates of the three genes with the specifically targeted SgRNA sequences are respectively 56%, 63%, and 41%. A three genes knockoutpig can be obtained, wherein the three genes related to immune rejectionare knocked out, and heart valves of said pig can be acquired.