公开(公告)号：US20240200126A1

公开(公告)日：2024-06-20

申请号：US18287245

申请日：2022-04-20

申请人： NANJING GENSCRIPT BIOTECH CO., LTD.

发明人： Feichi HU ,  Qi WANG ,  Cheng-Hsien WU ,  Nileshi SARAF

IPC分类号： C12Q1/6858 ,  C12Q1/6876

CPC分类号： C12Q1/6858 ,  C12Q1/6876

摘要： A primer group and method for detecting single-base mutations. The primer group comprises the following primers: an identification primer, which is composed of, from the 5′ end to the 3′ end, (a) a nucleotide sequence which complements a segment of continuous nucleotides in a nucleic acid sequence to be detected, wherein the 5′ end of the continuous nucleotides starts at a first nucleotide downstream of an expected mutation site; and (b) a nucleotide which complements a non-mutated nucleotide or an expected mutated nucleotide at the expected single-base mutation site of the nucleic acid sequence. The primer group also comprises an amplification primer. The amplification primer is capable of using the identification primer to amplify an amplification product which is obtained by amplifying the nucleic acid sequence. The identification primer is 1 to 19 nucleotides less than the amplification primer.