公开(公告)号：US3660240A

公开(公告)日：1972-05-02

申请号：US3660240D

申请日：1969-05-05

Applicant: NASA

Inventor： CHAPPELLE EMMETT W ,  PICCIOLO GRACE L

IPC: C12Q1/66 ,  G01N31/14

CPC classification number: C12Q1/66 ,  Y10S435/822 ,  Y10S435/832 ,  Y10S435/84 ,  Y10S435/849 ,  Y10S435/85 ,  Y10S435/852 ,  Y10S435/862 ,  Y10S435/873 ,  Y10S435/875 ,  Y10S435/876 ,  Y10S435/881 ,  Y10S435/883 ,  Y10S435/909

Abstract: Flavin coenzymes, the active forms of vitamin B2, are assayed by using boiling perchloric acid to rupture bacterial cells, free the flavin from protein, and hydrolyze flavin adenine dinucleotide to flavin mononucleotide, which is reduced with sodium borohydride and palladium chloride and reacted with the enzyme luciferase to produce light with intensity directly proportional to flavin concentration.

Abstract translation: 通过使用沸腾的高氯酸破碎细菌细胞，从蛋白质中释放黄素并将黄素腺嘌呤二核苷酸水解成黄素单核苷酸，用硼氢化钠和氯化钯还原并与其反应，测定黄素辅酶活性形式的维生素B2 酶荧光素酶产生与黄素浓度成正比的强度。