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    Detection of fish spoilage by colorimetry
    2.
    发明授权
    Detection of fish spoilage by colorimetry 失效
    通过比色法检测鱼腐败

    公开(公告)号：US5744321A

    公开(公告)日：1998-04-28

    申请号：US803946

    申请日：1997-02-21

    申请人： Patrick Harewood

    发明人： Patrick Harewood

    IPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/00 ,  C12Q1/02

    CPC分类号： C12Q1/06 ,  Y10S435/874 ,  Y10S435/875 ,  Y10S435/876 ,  Y10S435/877

    摘要： A rapid, on-site method for indicating the degree of spoilage, if any, of finfish by the level of bacteria present therein. A small quantity of flesh is cut from a representative fish and kneaded in a bacterial nutrient broth to extract any bacteria present. A triphenyl tetrazolium dye is added as an indicator reagent, followed by an anionic surfactant and a lower alkyl alcohol. The developed color, if any, is compared to a control color chart representative of acceptable and unsatisfactory degrees of bacterial contamination or spoilage.

    摘要翻译： 一种快速的现场方法，用于通过其中存在的细菌水平来指示鱼类的腐败程度（如果有的话）。 从代表性的鱼切割少量的肉，并在细菌营养肉汤中捏合以提取任何存在的细菌。 加入三苯基四唑鎓染料作为指示剂，然后加入阴离子表面活性剂和低级烷基醇。 将显影的颜色（如果有的话）与表示可接受和不令人满意的细菌污染或腐败程度的对照色图进行比较。

    Process for biotechnological upgrading of shale oil
    4.
    发明授权
    Process for biotechnological upgrading of shale oil 失效
    生物技术升级页岩油的方法

    公开(公告)号：US5049499A

    公开(公告)日：1991-09-17

    申请号：US298355

    申请日：1989-01-19

    申请人： Ronald M. Atlas ,  Jackie Aislabie

    发明人： Ronald M. Atlas ,  Jackie Aislabie

    IPC分类号： C10G32/00 ,  C12P1/04

    CPC分类号： C12P1/04 ,  C10G32/00 ,  Y10S435/875 ,  Y10S435/876

    摘要： A process for biotechnological upgrading of shale oil in selectively removing damaging nitrogen-containing compounds comprising treating the raw shale oil with special microbial cultures having specific ability to degrade the harmful nitrogen-containing compounds, such as the amines, nitriles and heterocyclics as the quinolines and pyridines, and converting them into non-damaging components.

    摘要翻译： 一种生物技术升级页岩油的选择性除去有害的含氮化合物的方法，包括用特殊的微生物培养物处理生页岩油，该微生物培养物具有降解有害的含氮化合物如胺，腈和杂环作为喹啉的特异性能力， 并将其转化为非破坏性组分。

    Method for microbial cell killing
    5.
    发明授权
    Method for microbial cell killing 失效
    微生物细胞杀伤方法

    公开(公告)号：US4966844A

    公开(公告)日：1990-10-30

    申请号：US771883

    申请日：1985-09-03

    申请人： Michael J. Tallen ,  Dennis M. Fenton

    发明人： Michael J. Tallen ,  Dennis M. Fenton

    IPC分类号： C07K14/55 ,  C12N1/00 ,  C12P1/00

    CPC分类号： C12N1/005 ,  C07K14/55 ,  C12P1/00 ,  Y10S435/80 ,  Y10S435/839 ,  Y10S435/849 ,  Y10S435/875

    摘要： In processes for recovery of biologically active polypeptides from fermentation cultures of recombinant host organisms, cell death is frequently a prerequisite for isolation processing of the recombinant product outside the fermentation vessel. Disclosed are improved methods for effecting efficient host cell death inside the fermentation vessel through uniformly contacting host cells in culture with microbicidal concentrations of benzyl alcohol. Illustratively, E. coli, B. subtilis, and P. aeruginosa cultures are advantageously treated with from 0.5 to 10.0% (v/v) of benzyl alcohol in the absence of pH or temperature changes within the fermentor.

    摘要翻译： 在从重组宿主生物的发酵培养物回收生物活性多肽的方法中，细胞死亡通常是发酵容器外的重组产物的分离处理的先决条件。 公开了通过使培养物中的宿主细胞与杀菌剂浓度的苯甲醇均匀接触来实现发酵容器内有效的宿主细胞死亡的改进方法。 示例性地，在发酵罐内没有pH或温度变化的情况下，有利地用0.5至10.0％（v / v）的苄醇处理大肠杆菌，枯草芽孢杆菌和铜绿假单胞菌培养物。

    Toxoids of elastase of Pseudomonas aeruginosa origin
    6.
    发明授权
    Toxoids of elastase of Pseudomonas aeruginosa origin 失效
    铜绿假单胞菌来源的弹性蛋白酶的毒素

    公开(公告)号：US4575459A

    公开(公告)日：1986-03-11

    申请号：US694637

    申请日：1985-01-24

    申请人： Yuzuru Homma ,  Kazuyuki Morihara

    发明人： Yuzuru Homma ,  Kazuyuki Morihara

    IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K16/12 ,  C07K16/40 ,  C12N9/66 ,  A61K39/104 ,  A61K39/40 ,  C12P21/00

    CPC分类号： C12N9/6448 ,  C07K16/1214 ,  C07K16/40 ,  A61K38/00 ,  A61K39/00 ,  Y10S435/875

    摘要： Provided herein are two kinds of toxoid of elastase of Pseudomonas aeruginosa origin, one of which is obtained by treating a purified elastase produced from Pseudomonas aeruginosa with a synthetic peptide for chloroacetyl-N-hydroxy-L-leucyl-L-alanylglycinamide and the other of which is obtained by treating at first with formalin, then, with the synthetic peptide. The present invention also contemplates a method for preparing the toxoids and the use of such toxoids for preventing and treating infections caused by Pseudomonas aeruginosa on human beings and mammalian animals. Acute toxicity of the toxoids is inspected.

    摘要翻译： 本文提供两种铜绿假单胞菌来源的弹性蛋白酶类毒素，其中之一是通过用氯乙酰基-N-羟基-L-亮氨酰-L-丙氨酰氨基磺酰胺的合成肽处理由绿脓假单胞菌产生的纯化弹性蛋白酶获得，另一种 首先用福尔马林处理，然后用合成肽获得。 本发明还考虑了一种制备类毒素的方法以及使用这种类毒素来预防和治疗由铜绿假单胞菌对人类和哺乳动物造成的感染。 检查毒素的急性毒性。

    Process for preparing acyl-CoA synthetase LCF-18
    7.
    发明授权
    Process for preparing acyl-CoA synthetase LCF-18 失效
    制备酰基辅酶A合成酶LCF-18的方法

    公开(公告)号：US4229538A

    公开(公告)日：1980-10-21

    申请号：US28604

    申请日：1979-04-09

    申请人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

    发明人： Hideaki Yamada ,  Sakayu Shimizu ,  Yoshiki Tani

    IPC分类号： C12N9/00 ,  C12Q1/25 ,  C12N9/10

    CPC分类号： C12N9/93 ,  C12Q1/25 ,  Y10S435/875 ,  Y10S435/911

    摘要： Acyl-CoA synthetase, having a high activity to C.sub.16 -C.sub.18 long chain fatty acids, is obtained by culturing strains belonging to various genera. As is known, acyl-CoA synthetase, having a strong activity to C.sub.16 -C.sub.18 long chain fatty acids, is generally obtained from liver of rat; however, it has now been discovered that acyl-CoA synthetase can be obtained from microorganisms and this enzyme is called acyl-CoA synthetase LCF-18. By use of acyl-CoA synthetase LCF-18 of the present invention which has a high activity to C.sub.16 -C.sub.18 long chain fatty acids, serum non-esterified fatty acid of human beings can be accurately determined, and it is very useful for diagnosis of diabetes and so forth.

    摘要翻译： 通过培养属于各种属的菌株获得对C16-C18长链脂肪酸具有高活性的酰基辅酶A合成酶。 众所周知，对C16-C18长链脂肪酸具有强烈活性的酰基辅酶A合成酶通常由大鼠肝脏获得; 然而，现在已经发现可以从微生物获得酰基辅酶A合成酶，该酶称为酰基辅酶A合成酶LCF-18。 通过使用对C16-C18长链脂肪酸具有高活性的本发明的酰基-CoA合成酶LCF-18，可以准确测定人的血清非酯化脂肪酸，并且其对于诊断 糖尿病等。

    String culture inoculum
    9.
    发明授权
    String culture inoculum 失效
    菌丝培养接种物

    公开(公告)号：US4021305A

    公开(公告)日：1977-05-03

    申请号：US687724

    申请日：1976-05-19

    申请人： Robert D. Muir ,  Vijay K. Sangar

    发明人： Robert D. Muir ,  Vijay K. Sangar

    IPC分类号： C12N1/20 ,  C12B1/02 ,  C12K3/00

    CPC分类号： C12N1/20 ,  Y10S435/849 ,  Y10S435/852 ,  Y10S435/873 ,  Y10S435/875 ,  Y10S435/879 ,  Y10S435/881 ,  Y10S435/882 ,  Y10S435/883 ,  Y10S435/884

    摘要： The present invention is concerned with a string culture inoculum comprising a string having stabilized cells of one of______________________________________ Salmonella typhimurium ATCC 6994 Klebsiella pneumoniae ATCC 10031 Enterobacter cloacae ATCC 11367 Escherichia coli ATCC 25922 Citrobacter freundii ATCC 6750 Proteus vulgaris ATCC 881 Pseudomonas aeruginosa ATCC 15442 Shigella sonnei ATCC 11060 Serratia marcescens ATCC 14756 Staphylococcus aureus ATCC 25923 Staphylococcus epidermidis ATCC 17917 Streptococcus faicalis ATCC 10541 Streptococcus pyogenes ATCC 12202 ______________________________________ dispersed throughout, said string culture inoculum having a sufficient number of stabilized cells adhered thereto to produce at least 10.sup.5 cells per milliliter upon incubation in 3 milliliters of suitable broth at 37.degree. C. for 2 hours. The string culture inocula of the present invention are useful as quality control cultures for positive as well as negative controls in clinical bacteriology procedures and drug susceptibility testing.

    摘要翻译： 本发明涉及包含具有稳定的细胞的菌株的串培养接种物，其具有以下菌株之一：鼠伤寒沙门氏菌ATCC 6994}肺炎克雷伯杆菌ATCC 10031}阴沟肠杆菌ATCC 11367}大肠杆菌ATCC 25922}弗氏弗曲达曲霉ATCC 6750}普通变形杆菌ATCC 881} 铜绿假单胞菌ATCC 15442}志贺氏杆菌ATCC 11060}粘质沙雷氏菌ATCC14756}金黄色葡萄球菌ATCC 25923}表皮葡萄球菌ATCC 17917}猪链球菌ATCC 10541}化脓性链球菌ATCC12202}，所述串培养接种物具有足够数量的稳定细胞粘附 在3毫升合适的肉汤中于37℃孵育2小时后产生至少105个细胞/毫升。 本发明的菌丝培养接种物可用作临床细菌学方法和药物敏感性试验中阳性和阴性对照的质量控制培养物。

    Process for producing high-molecular nucleic acids by fermentation
    10.
    发明授权
    Process for producing high-molecular nucleic acids by fermentation 失效
    通过发酵生产高分子核酸的方法

    公开(公告)号：US3880738A

    公开(公告)日：1975-04-29

    申请号：US36703673

    申请日：1973-06-04

    申请人： KYOWA HAKKO KOGYO KK

    发明人： SUZUKI TAKEO ,  TOMITA FUSAO ,  ITO SEIGA

    IPC分类号： C12P19/34 ,  C12D13/06

    CPC分类号： C12P19/34 ,  Y10S435/83 ,  Y10S435/84 ,  Y10S435/843 ,  Y10S435/859 ,  Y10S435/875 ,  Y10S435/918 ,  Y10S435/923

    摘要： The present invention relates to a process for producing deoxyribonucleic acids and/or ribonucleic acids (hereinafter designated as high-molecular nucleic acids), which is characterized by culturing aerobically a microorganism capable of producing deoxyribonucleic acid and/or ribonucleic acid in a culture medium containing hydrocarbon or carbohydrate as main carbon source, accumulating a substantial amount of deoxyribonucleic acid and/or ribonucleic acid and recovering the accumulated deoxyribonucleic acid and/or ribonucleic acid from the fermentation liquor.

    摘要翻译： 本发明涉及一种生产脱氧核糖核酸和/或核糖核酸（以下称为高分子核酸）的方法，其特征在于在含有以下物质的培养基中有氧地培养能够产生脱氧核糖核酸和/或核糖核酸的微生物 碳氢化合物或碳水化合物作为主要碳源，积累大量脱氧核糖核酸和/或核糖核酸，并从发酵液中回收积累的脱氧核糖核酸和/或核糖核酸。