公开(公告)号：US20140069849A1

公开(公告)日：2014-03-13

申请号：US13969704

申请日：2013-08-19

Applicant: National Institute of Advanced Industrial Science and Technology

Inventor： Masaya MIYAZAKI ,  Daisuke SUGIYAMA ,  Kenichi YAMASHITA ,  Yuuki TESHIMA ,  Hiroyuki NAKAMURA ,  Hideaki MAEDA

IPC: B03B5/00

CPC classification number: C12M47/04 ,  B01L3/502761 ,  B01L2300/0864 ,  B03B5/28 ,  B03B2005/405

Abstract: Provided are a particle separation device and a method of separating particles, each of which can easily separate particles for a short time without a great stress to the particles and easily collect the particles thus separated. The particle separation device includes at least: a separation flow path (i) to which a separation fluid and a particle group including two types of particles being different in density are supplied, and (ii) through which the separation fluid flows in a certain direction; a divergence section connected to at least an end of the separation flow path in the certain direction in which the separation fluid flows, a first flow path formed from the divergence section in a rising direction; and a second flow path formed from the divergence section in a falling direction, the separation flow path being formed in a horizontal direction.

Abstract translation: 提供了一种颗粒分离装置和分离颗粒的方法，每个颗粒可以在短时间内容易地分离颗粒，而不会对颗粒产生大的应力，并且容易地收集由此分离的颗粒。 颗粒分离装置至少包括：供给分离流体和包含两种密度不同的颗粒的颗粒群的分离流路（i），（ii）分离流体沿着某一方向流动 ; 分离部，其在分离流体流动的一定方向上与分离流路的至少一端连接，第一流路沿着上升方向从发散部形成; 以及由所述发散部沿下落方向形成的第二流路，所述分离流路沿水平方向形成。

公开(公告)号：US20160017273A1

公开(公告)日：2016-01-21

申请号：US14772623

申请日：2014-01-06

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Kenichi YAMASHITA ,  Maria Portia NAGATA ,  Masaya MIYAZAKI ,  Hideaki MAEDA

IPC: C12M1/00 ,  C12N5/071

CPC classification number: C12M47/04 ,  C12N5/0612

Abstract: A sperm selection unit structure for efficiently separating sperm having favorable motility includes: a storage part which stores semen acquired from an animal therein; a buffer solution flow passage which communicates with the storage part and in which a buffer solution flows in the direction toward the storage part as a laminar flow; and a swim-up passage which communicates with the storage part such that the sperm swim up in the buffer solution flow passage from the storage part, wherein a downstream part of the swim-up passage is formed of a flow passage having a wide width where the buffer solution flows as a slow flow while maintaining a laminar flow state of the buffer solution, and a downstream end portion of the flow passage is opened in a state where the downstream end portion faces a wall surface of the storage part.

Abstract translation: 用于有效分离具有良好运动性的精子的精子选择单元结构包括：存储从动物获取的精液的储存部分; 缓冲溶液流动通道，其与所述储存部分连通，并且其中缓冲溶液以朝向所述存储部分的方向作为层流流动; 以及游泳通道，其与储存部分连通，使得精子从储存部分在缓冲溶液流动通道中游泳，其中游泳通道的下游部分由宽度宽的流动通道形成， 缓冲溶液以缓慢的流动流动，同时保持缓冲溶液的层流状态，并且在下游端部分面向存储部分的壁表面的状态下，打开流道的下游端部。