公开(公告)号：US20240035001A1

公开(公告)日：2024-02-01

申请号：US18356448

申请日：2023-07-21

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  AJINOMOTO CO., INC.

发明人： Keishi KAMEYAMA ,  Hideyuki TAMAKI ,  Hiroyuki KUSADA ,  Yoichi KAMAGATA

IPC分类号： C12N7/00 ,  C12Q1/04 ,  C12N9/36 ,  A61K35/76

CPC分类号： C12N7/00 ,  C12Q1/04 ,  C12N9/2462 ,  C12Y302/01017 ,  A61K35/76 ,  C12N2795/14071

摘要： A bacteriophage which consists of the nucleotide sequence represented by SEQ ID NO: 1 or a nucleotide sequence having 90% or more identity thereto, as its genome, is capable of infecting and lysing a diabetes-inducible bacterium belonging to Fusimonas intestini,containing a cyclic single-stranded DNA.

公开(公告)号：US20220307063A1

公开(公告)日：2022-09-29

申请号：US17615853

申请日：2020-06-24

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Shunsuke TOMITA ,  Ryoji KURITA ,  Hideyuki TAMAKI ,  Hiroyuki KUSADA ,  Naoshi KOJIMA ,  Sayaka ISHIHARA ,  Koyomi MIYAZAKI ,  Isao YUMOTO

IPC分类号： C12Q1/04 ,  G01N21/64

摘要： A method for analyzing a microbiota, comprising (1) dissolving a probe capable of non-specifically interacting with a plurality of microorganisms in a plurality of solvents having different ionic strengths and pH levels, wherein the probe comprises: (a) a cationic polymer and (b) an environment-sensitive fluorophore; (2) adding a test sample containing the microbiota to a plurality of probe solutions prepared in the step (1), thereby microorganisms in the test sample and the probe are interacted non-specifically; (3) measuring fluorescence intensities of the plurality of probe solutions to which the test sample has been added in the step (2); and (4) comparing the pattern of fluorescence intensities obtained in the step (3) with the pattern of fluorescence intensities obtained from a reference sample.