公开(公告)号：US10066199B2

公开(公告)日：2018-09-04

申请号：US14650560

申请日：2013-12-12

申请人： NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY

发明人： Akira Mizuno ,  Rika Numano ,  Hirofumi Kurita

IPC分类号： C12M1/42 ,  C12N15/87 ,  C12N13/00

摘要： An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells by electrical action and a method for manufacturing exogenous substance-bearing cells at low cost are provided.In the introducing an exogenous substance like a gene into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container through an opening portion in an amount that is prepared such that a liquid droplet that is formed will not come into contact with a pair of electrodes simultaneously. The liquid droplet is formed in an oil stored in the container, without the cell suspension liquid mixing with the oil. When a direct current voltage is supplied to the electrodes from a power supply, the liquid droplet moves there-between, and the exogenous substance is introduced into the cell within the liquid droplet by electrical action.

公开(公告)号：US10082453B2

公开(公告)日：2018-09-25

申请号：US15122055

申请日：2014-09-19

申请人： LG ELECTRONICS INC. ,  NATIONAL UNIVERSITY CORPORATION TOYOHASHI UNIVERSITY OF TECHNOLOGY

发明人： Chulwoo Park ,  Sunghwa Lee ,  Akira Mizuno ,  Kazunori Takashima ,  Hirofumi Kurita ,  Hachiro Yasuda

IPC分类号： G01N15/06 ,  G01N21/76 ,  C12Q1/04 ,  G01N21/84 ,  G01N15/00 ,  G01N21/64

CPC分类号： G01N15/0606 ,  C12Q1/04 ,  G01N15/0637 ,  G01N21/6428 ,  G01N21/76 ,  G01N21/8483 ,  G01N2015/0069 ,  G01N2021/6439

摘要： An airborne microbial measurement apparatus and a measurement method thereof are provided. An airborne microbial measurement apparatus according to an embodiment includes a discharge apparatus including a discharge electrode and a voltage supply unit applying a high voltage to the discharge electrode. A substrate is provided to a side of the discharge apparatus to collect an airborne microbe from air by a high voltage applied to the discharge electrode. A reagent injection apparatus supplies a dyeing reagent to the microbe collected on the substrate or a DNA of the microbe. A light emission measurement apparatus senses a quantity of light generated from the DNA to which the dyeing reagent is supplied. The discharge apparatus includes a controller controlling the voltage supply unit so that the voltage is applied to collect the airborne microbe or destroy an external wall of the collected airborne microbe.