公开(公告)号：US20250136635A1

公开(公告)日：2025-05-01

申请号：US18835547

申请日：2023-02-06

Applicant: Nitto Denko Corporation

Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

IPC: C07H21/02

Abstract: Provided herein is a method for suppressing the amount of nucleoside phosphoramidites remaining in a reactor and reducing the amount of nucleoside phosphoramidites used in a synthesis step in a method for producing an oligonucleotide. The above problem was solved by a method for producing an oligonucleotide, wherein: the method includes binding a nucleoside phosphoramidite to a hydroxyl group, thiol group, or amino group at the 3′ or 5′ position of a nucleoside supported directly or indirectly on a carrier in the presence of an activating agent; and the amount of activating agent used in the binding is a 10.0 to 15.0-fold equivalent of the amount of nucleoside phosphoramidite used in the binding.

公开(公告)号：US20250155852A1

公开(公告)日：2025-05-15

申请号：US18838420

申请日：2023-02-21

Applicant: NITTO DENKO CORPORATION

Inventor： Kei YOSHIDA ,  Yoichi KIGAWA ,  Satoshi MATSUOKA ,  Jun MATSUNAMI

IPC: G05B13/02

Abstract: A condition optimization device includes an actual reaction value obtainment unit to obtain an actual reaction value by observing a predetermined physical property value at a predetermined condition value; a condition value calculation unit to calculate a new condition value by Bayesian optimization; an optimum condition value prediction unit to predict, by a response surface method, a best physical property value and an optimum condition value at which the best physical property value is obtainable; a first determination unit to determine whether or not convergence has occurred based on the condition value corresponding to the actual reaction value and based on the new condition value; and a second determination unit to determine, upon the first determination unit determining that the convergence has occurred, whether or not convergence has occurred based on a difference between the physical property value observed at the optimum condition value and the best physical property value.

公开(公告)号：US20250145656A1

公开(公告)日：2025-05-08

申请号：US18835699

申请日：2023-02-06

Applicant: Nitto Denko Corporation

Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

IPC: C07H21/04 ,  C07H1/02 ,  C07H21/02

Abstract: Provided herein is a method for producing oligonucleotides, that curbs the amount of nucleoside phosphoramidites remaining in a reaction container in a synthesis step, and that reduces the usage amount of nucleoside phosphoramidites. This method for producing oligonucleotides includes: (a) detaching a protecting group from a protected nucleoside that is directly or indirectly supported on a carrier and that has the protecting group bonded to a hydroxyl group, a thiol group, or an amino group located at the 3′-position or the 5′-position; (b) in the presence of an activating reagent, forming a bond between a nucleoside phosphoramidite and the hydroxyl group, the thiol group, or the amino group located at the 3′-position or the 5′-position of the nucleoside from which the protecting group had been detached and which is directly or indirectly supported on the carrier; (c) sulfurizing or oxidizing the bond formed in the step (b); and (d) capping an unbound hydroxyl group, thiol group, or amino group located at the 3′-position or the 5′-position in the nucleoside directly or indirectly supported on the carrier.

公开(公告)号：US20240242778A1

公开(公告)日：2024-07-18

申请号：US18275076

申请日：2023-04-10

Applicant: Nitto Denko Corporation

Inventor： Kei YOSHIDA ,  Yoichi KIGAWA ,  Shinsuke SUGIURA ,  Eri MAETA ,  Jun MATSUNAMI

IPC: G16B40/10 ,  G01N21/3577 ,  G01N21/65 ,  G16B50/30

CPC classification number: G16B40/10 ,  G01N21/3577 ,  G01N21/65 ,  G16B50/30

Abstract: A nucleoside phosphoramidite identifying system for improving a nucleoside phosphoramidite identification accuracy includes a memory unit configured to store spectra of solutions of a plurality of different nucleoside phosphoramidites, a detecting unit configured to detect a spectrum of a solution of a nucleoside phosphoramidite, and an identifying unit configured to identify the nucleoside phosphoramidite based on cosine similarity between the spectra stored in the memory unit and the spectrum detected by the detecting unit.