公开(公告)号：US20250145656A1

公开(公告)日：2025-05-08

申请号：US18835699

申请日：2023-02-06

Applicant: Nitto Denko Corporation

Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

IPC: C07H21/04 ,  C07H1/02 ,  C07H21/02

Abstract: Provided herein is a method for producing oligonucleotides, that curbs the amount of nucleoside phosphoramidites remaining in a reaction container in a synthesis step, and that reduces the usage amount of nucleoside phosphoramidites. This method for producing oligonucleotides includes: (a) detaching a protecting group from a protected nucleoside that is directly or indirectly supported on a carrier and that has the protecting group bonded to a hydroxyl group, a thiol group, or an amino group located at the 3′-position or the 5′-position; (b) in the presence of an activating reagent, forming a bond between a nucleoside phosphoramidite and the hydroxyl group, the thiol group, or the amino group located at the 3′-position or the 5′-position of the nucleoside from which the protecting group had been detached and which is directly or indirectly supported on the carrier; (c) sulfurizing or oxidizing the bond formed in the step (b); and (d) capping an unbound hydroxyl group, thiol group, or amino group located at the 3′-position or the 5′-position in the nucleoside directly or indirectly supported on the carrier.

公开(公告)号：US20170002038A1

公开(公告)日：2017-01-05

申请号：US15185875

申请日：2016-06-17

Applicant: Nitto Denko Corporation

Inventor： Shohei HORIE ,  Tatsuya KONISHI ,  Kenjiro MORI ,  Eri MAETA ,  Tsuyoshi MUKOBATA ,  Masafumi IWAMOTO

IPC: C07H21/02 ,  C07H1/06

CPC classification number: C07H21/02 ,  C07H1/06 ,  C12N15/1006

Abstract: The invention provides a cut-out method capable of suppressing production of a byproduct, wherein the object RNA oligonucleotide is not cleaved from a universal linker, which is produced in cutting out the object RNA oligonucleotide from the universal support, and capable of increasing the yield of the object RNA oligonucleotide. The RNA oligonucleotide cut-out method includes a step of bringing a universal support supporting an RNA oligonucleotide in contact with an aqueous solution containing alkylamine and a monovalent inorganic salt.

Abstract translation: 本发明提供能够抑制副产物生成的切断方法，其中目标RNA寡核苷酸不从通用接头切割，该通用接头是从通用载体切出目标RNA寡核苷酸而产生的，并且能够提高产率 的目标RNA寡核苷酸。 RNA寡核苷酸切割方法包括使支持RNA寡核苷酸的通用载体与含有烷基胺和一价无机盐的水溶液接触的步骤。

公开(公告)号：US20250136635A1

公开(公告)日：2025-05-01

申请号：US18835547

申请日：2023-02-06

Applicant: Nitto Denko Corporation

Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

IPC: C07H21/02

Abstract: Provided herein is a method for suppressing the amount of nucleoside phosphoramidites remaining in a reactor and reducing the amount of nucleoside phosphoramidites used in a synthesis step in a method for producing an oligonucleotide. The above problem was solved by a method for producing an oligonucleotide, wherein: the method includes binding a nucleoside phosphoramidite to a hydroxyl group, thiol group, or amino group at the 3′ or 5′ position of a nucleoside supported directly or indirectly on a carrier in the presence of an activating agent; and the amount of activating agent used in the binding is a 10.0 to 15.0-fold equivalent of the amount of nucleoside phosphoramidite used in the binding.

公开(公告)号：US20240391945A1

公开(公告)日：2024-11-28

申请号：US17791314

申请日：2021-01-07

Applicant: NITTO DENKO CORPORATION ,  NITTO DENKO AVECIA, INC.

Inventor： Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Kota SAKAMOTO ,  Eduardo PAREDES

IPC: C07H21/00 ,  C07H1/00

Abstract: The purpose of the present invention is to provide a method for synthesizing an oligonucleotide by using a segment-type amidite. An oligonucleotide production method comprising at least one coupling step for coupling a nucleoside phosphoramidite with a thiol group or a hydroxyl group at the 3′ or 5′ of a nucleoside or a nucleotide in the presence of an activator, wherein, in the at least one coupling step, said nucleoside phosphoramidite has (a) two or more nucleoside portions or (b) at least one nucleoside portion and a linker portion, and said activator has a structure represented by formula (1) (in formula (1), X represents an organic base) or by formula (2) (in formula (2), R1 and R2 are each independently selected from the group consisting of H, straight chain or branched chain C1-7 alkyl groups and optionally substituted aromatic groups).