中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

8 records (took 0.014 seconds)

    METHOD FOR PRODUCING OLIGONUCLEOTIDES
    1.
    发明申请
    METHOD FOR PRODUCING OLIGONUCLEOTIDES 有权

    公开(公告)号：US20250145656A1

    公开(公告)日：2025-05-08

    申请号：US18835699

    申请日：2023-02-06

    Applicant: Nitto Denko Corporation

    Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

    IPC: C07H21/04 ,  C07H1/02 ,  C07H21/02

    Abstract: Provided herein is a method for producing oligonucleotides, that curbs the amount of nucleoside phosphoramidites remaining in a reaction container in a synthesis step, and that reduces the usage amount of nucleoside phosphoramidites. This method for producing oligonucleotides includes: (a) detaching a protecting group from a protected nucleoside that is directly or indirectly supported on a carrier and that has the protecting group bonded to a hydroxyl group, a thiol group, or an amino group located at the 3′-position or the 5′-position; (b) in the presence of an activating reagent, forming a bond between a nucleoside phosphoramidite and the hydroxyl group, the thiol group, or the amino group located at the 3′-position or the 5′-position of the nucleoside from which the protecting group had been detached and which is directly or indirectly supported on the carrier; (c) sulfurizing or oxidizing the bond formed in the step (b); and (d) capping an unbound hydroxyl group, thiol group, or amino group located at the 3′-position or the 5′-position in the nucleoside directly or indirectly supported on the carrier.

    SOLID-PHASE SUPPORT FOR OLIGONUCLEOTIDE SYNTHESIS AND OLIGONUCLEOTIDE SYNTHESIS METHOD
    3.
    发明申请
    SOLID-PHASE SUPPORT FOR OLIGONUCLEOTIDE SYNTHESIS AND OLIGONUCLEOTIDE SYNTHESIS METHOD 有权
    寡核苷酸合成和寡核苷酸合成方法的固相支持

    公开(公告)号：US20130317206A1

    公开(公告)日：2013-11-28

    申请号：US13901266

    申请日：2013-05-23

    Applicant: Nitto Denko Corporation

    Inventor： Eri MAETA ,  Kenjiro MORI ,  Kazuya MIWA

    IPC: C08F22/10 ,  C07H21/04

    CPC classification number: C08F22/105 ,  C07B35/04 ,  C07B61/02 ,  C07H19/04 ,  C07H19/06 ,  C07H19/16 ,  C07H21/04 ,  C08F2/18 ,  C08F8/12 ,  C08F12/22 ,  C08F212/08 ,  C08F212/36 ,  C08F2800/20 ,  C08F2220/286 ,  C08F212/14

    Abstract: The present invention provides a solid-phase support for oligonucleotide synthesis for synthesizing long chain oligonucleotide, RNA oligonucleotide and modified oligonucleotide at high synthetic quantity and high purity with a low loading amount of a linker. Provided is a solid-phase support for oligonucleotide synthesis comprising a porous resin bead having a monovinyl monomer unit, a crosslinkable vinyl monomer unit and a polyethylene glycol unit and a cleavable linker loaded on its surface, the porous resin bead having a group capable of binding to a carboxy group by a dehydration condensation reaction on its surface, the cleavable linker having a carboxy group, wherein the carboxy group of the cleavable linker is bound to the group capable of binding to a carboxy group, by a dehydration condensation reaction, and a loading amount of the cleavable linker is 1 to 80 μmol/g relative to the weight of the porous resin bead.

    Abstract translation: 本发明提供了一种用于合成高链寡核苷酸，RNA寡核苷酸和高合成量和高纯度的具有低负载量的接头的高纯度的修饰的寡核苷酸的寡核苷酸合成的固相支持物。 提供了一种用于寡核苷酸合成的固相载体，其包含具有单乙烯基单体单元，可交联乙烯基单体单元和聚乙二醇单元的多孔树脂珠粒和其表面上负载的可切割连接物，所述多孔树脂珠粒具有能够结合的基团 通过其表面上的脱水缩合反应与羧基反应，可切割接头具有羧基，其中可切割接头的羧基通过脱水缩合反应与能结合羧基的基团结合，并且 可切割接头的负载量相对于多孔树脂珠的重量为1〜80μmol/ g。

    METHOD OF CUTTING OUT RNA OLIGONUCLEOTIDE
    5.
    发明申请
    METHOD OF CUTTING OUT RNA OLIGONUCLEOTIDE 审中-公开
    切出RNA寡核苷酸的方法

    公开(公告)号：US20170002038A1

    公开(公告)日：2017-01-05

    申请号：US15185875

    申请日：2016-06-17

    Applicant: Nitto Denko Corporation

    Inventor： Shohei HORIE ,  Tatsuya KONISHI ,  Kenjiro MORI ,  Eri MAETA ,  Tsuyoshi MUKOBATA ,  Masafumi IWAMOTO

    IPC: C07H21/02 ,  C07H1/06

    CPC classification number: C07H21/02 ,  C07H1/06 ,  C12N15/1006

    Abstract: The invention provides a cut-out method capable of suppressing production of a byproduct, wherein the object RNA oligonucleotide is not cleaved from a universal linker, which is produced in cutting out the object RNA oligonucleotide from the universal support, and capable of increasing the yield of the object RNA oligonucleotide. The RNA oligonucleotide cut-out method includes a step of bringing a universal support supporting an RNA oligonucleotide in contact with an aqueous solution containing alkylamine and a monovalent inorganic salt.

    Abstract translation: 本发明提供能够抑制副产物生成的切断方法，其中目标RNA寡核苷酸不从通用接头切割，该通用接头是从通用载体切出目标RNA寡核苷酸而产生的，并且能够提高产率 的目标RNA寡核苷酸。 RNA寡核苷酸切割方法包括使支持RNA寡核苷酸的通用载体与含有烷基胺和一价无机盐的水溶液接触的步骤。

    METHOD FOR PRODUCING OLIGONUCLEOTIDE
    7.
    发明申请
    METHOD FOR PRODUCING OLIGONUCLEOTIDE 有权

    公开(公告)号：US20250136635A1

    公开(公告)日：2025-05-01

    申请号：US18835547

    申请日：2023-02-06

    Applicant: Nitto Denko Corporation

    Inventor： Kei YOSHIDA ,  Tomoyoshi IIDA ,  Masafumi IWAMOTO ,  Eri MAETA ,  Jun MATSUNAMI

    IPC: C07H21/02

    Abstract: Provided herein is a method for suppressing the amount of nucleoside phosphoramidites remaining in a reactor and reducing the amount of nucleoside phosphoramidites used in a synthesis step in a method for producing an oligonucleotide. The above problem was solved by a method for producing an oligonucleotide, wherein: the method includes binding a nucleoside phosphoramidite to a hydroxyl group, thiol group, or amino group at the 3′ or 5′ position of a nucleoside supported directly or indirectly on a carrier in the presence of an activating agent; and the amount of activating agent used in the binding is a 10.0 to 15.0-fold equivalent of the amount of nucleoside phosphoramidite used in the binding.

Patent Agency Ranking