公开(公告)号：US20220235341A1

公开(公告)日：2022-07-28

申请号：US17435719

申请日：2020-03-20

申请人： Novozymes A/S

发明人： Carsten Andersen ,  Sofia Arnehed ,  Lars Lehmann Hylling Christensen ,  Ali Fallah-Araghi ,  Markus Klinger ,  Anders Gunnar Sandstrom

IPC分类号： C12N9/26 ,  C11D11/00 ,  C11D3/386

摘要： The present invention relates to alpha-amylase variants of a parent alpha-amylase wherein said alpha-amylase variants have an improved wash performance as compared to said parent alpha-amylase. The invention also relates to compositions comprising the variants of the invention, uses of said variants and methods of producing said variants.

公开(公告)号：US10883102B2

公开(公告)日：2021-01-05

申请号：US15560017

申请日：2016-03-15

申请人： Novozymes A/S

发明人： Ali Fallah-Araghi ,  Ole Skyggebjerg

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： A method for the identification of a polynucleotide encoding an enzyme of interest in a microfluidic device by providing an emulsion of microfluidic droplets comprising a library of polynucleotides encoding one or more enzyme, introducing a concentrated PCR solution which allows PCR amplification of the polynucleotides into selected droplets and/or introducing a lethal solution into deselected droplets, and identifying a polynucleotide encoding an enzyme of interest.

公开(公告)号：US20170240877A1

公开(公告)日：2017-08-24

申请号：US15317881

申请日：2015-06-12

申请人： NOVOZYMES A/S

发明人： Carsten Andersen ,  Signe Eskildsen Larsen ,  Ali Fallah-Araghi

IPC分类号： C12N9/28 ,  C11D3/386

CPC分类号： C12N9/2417 ,  C11D3/386 ,  C11D3/38681 ,  C12Y302/01001

摘要： The present invention relates toalpha-amylasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US10428321B2

公开(公告)日：2019-10-01

申请号：US15317881

申请日：2015-06-12

申请人： NOVOZYMES A/S

发明人： Carsten Andersen ,  Signe Eskildsen Larsen ,  Ali Fallah-Araghi

IPC分类号： C12N9/28 ,  C11D3/386

摘要： The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20180094255A1

公开(公告)日：2018-04-05

申请号：US15560017

申请日：2016-03-15

申请人： Novozymes A/S

发明人： Ali Fallah-Araghi ,  Ole Skyggebjerg

IPC分类号： C12N15/10 ,  C12Q1/6806

CPC分类号： C12N15/1075 ,  C12Q1/6806 ,  C12Q2563/159

摘要： A method for the identification of a polynucleotide encoding an enzyme of interest in a microfluidic device by providing an emulsion of microfluidic droplets comprising a library of polynucleotides encoding one or more enzyme, introducing a concentrated PCR solution which allows PCR amplification of the polynucleotides into selected droplets and/or introducing a lethal solution into deselected droplets, and identifying a polynucleotide encoding an enzyme of interest.

公开(公告)号：US20170121696A1

公开(公告)日：2017-05-04

申请号：US15317872

申请日：2015-06-12

申请人： NOVOZYMES A/S

发明人： Carsten Andersen ,  Anne Dorte Houg ,  Signe Eskildsen Larsen ,  Ali Fallah-Araghi

IPC分类号： C12N9/28 ,  C11D11/00 ,  C11D3/386

CPC分类号： C12N9/2417 ,  C11D3/386 ,  C11D3/38681 ,  C11D11/0017 ,  C11D11/0023 ,  C12Y302/01001

摘要： The present invention relates to oxidation stable alpha-amylase variants. The present invention also relates to poly-nucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.