公开(公告)号：US20180355404A1

公开(公告)日：2018-12-13

申请号：US15563692

申请日：2016-04-07

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich ,  Allan Svendsen ,  Jesper Brask ,  Anne Olsen ,  Kathleen Hirano ,  Amy Twite

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  C12Q1/34

Abstract: Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.