公开(公告)号：US20190161786A1

公开(公告)日：2019-05-30

申请号：US16091765

申请日：2017-04-07

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich

IPC: C12Q1/37 ,  C12N15/10 ,  C40B50/06 ,  C40B20/02 ,  C40B40/08

Abstract: Provided herein are systems and components thereof for improving protease activity. The systems make use of an emulsion for in vitro compartmentalization of a library of synthetic compounds, each compound having a gene linked to a protease substrate and selectable marker. Expressed enzymes with greater protease activity will preferentially hydrolyze the protease substrate, whereas enzymes with less protease activity will leave the substrate intact. Removal of the non-hydrolyzed compounds provides an enriched gene library encoding for more active protease variants. Also described are synthetic compounds and emulsions which can be used in the methods.

公开(公告)号：US20180073017A1

公开(公告)日：2018-03-15

申请号：US15563707

申请日：2016-04-07

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich ,  Allan Svendsen

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1075 ,  C12N15/1058 ,  C12Q1/25 ,  C12Q1/6811 ,  C12Q1/6869

Abstract: Provided herein are methods and means for enhancing enzymatic activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a gene and a marked enzymatic substrate both directly linked to a solid phase. Expressed enzymes with greater activity will preferentially release the selectable marker from the solid phase, whereas enzymes with less activity will leave the markers intact. Removal of the marked compounds provides an enriched gene library encoding for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.

公开(公告)号：US20180355404A1

公开(公告)日：2018-12-13

申请号：US15563692

申请日：2016-04-07

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich ,  Allan Svendsen ,  Jesper Brask ,  Anne Olsen ,  Kathleen Hirano ,  Amy Twite

IPC: C12Q1/44

CPC classification number: C12Q1/44 ,  C12Q1/34

Abstract: Provided herein are methods and means for enhancing lipase activity. The system makes use of an emulsion for in vitro compartmentalization of a library of synthetic compounds which have a polynucleotide linked to a lipase substrate (e.g., a triglyceride). Expressed polypeptides having greater lipase activity will preferentially hydrolyze the substrate from the linked polynucleotide. Genes encoding polypeptides having less lipase activity will remain linked to the substrate and may be removed to enrich the library for more active variants. Also described are synthetic compounds and emulsions which can be used in the methods.

公开(公告)号：US20170369860A1

公开(公告)日：2017-12-28

申请号：US15538816

申请日：2016-01-06

Applicant: Novozymes A/S

Inventor： Robert Blazej ,  Charles Emrich ,  Nicholas Toriello ,  Hanshu Ding ,  David Osborn ,  Aubrey Jones

IPC: C12N9/42 ,  C12P19/14 ,  C12P5/02 ,  C12P5/00 ,  D21C5/00 ,  C12P7/40 ,  C12P3/00

CPC classification number: C12N9/2437 ,  C12P3/00 ,  C12P5/007 ,  C12P5/02 ,  C12P5/023 ,  C12P5/026 ,  C12P7/04 ,  C12P7/40 ,  C12P19/14 ,  C12Y302/01004 ,  D21C5/005 ,  D21H17/005 ,  Y02E50/343

Abstract: The present invention relates to endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, expression vectors, and recombinant host cells comprising the polynucleotides; and methods of using the variants.

公开(公告)号：US20150218553A1

公开(公告)日：2015-08-06

申请号：US14429117

申请日：2013-09-20

Applicant: NOVOZYMES A/S

Inventor： Robert Blazej ,  Nicholas Toriello ,  Charles Emrich

IPC: C12N15/10

CPC classification number: C12N15/1086 ,  C12N15/1075

Abstract: The present invention provides methods based on screening expressed polynucleotide libraries for soluble proteins.

Abstract translation: 本发明提供了基于筛选表达的可溶性蛋白质的多核苷酸文库的方法。