公开(公告)号：US20230012672A1

公开(公告)日：2023-01-19

申请号：US17642925

申请日：2020-09-16

Applicant: Novozymes A/S

Inventor： Hong Zhi Huang ,  Jiyin Liu ,  Hui Xu ,  Elena Vlasenko ,  Brett McBrayer ,  Ye Liu ,  Lan Tang ,  James Ron Huffman ,  Nikolaj Spodsberg

IPC: C12N9/24 ,  C12N1/18 ,  C12N15/81 ,  C12P7/06

Abstract: The present invention relates to isolated polypeptides having beta-glucanase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules. The present invention further relates to processes for producing fermentation products from starch-containing or cellulosic-containing material, as well as an enzyme blend or composition, or a recombinant host cell or fermenting organism suitable for use in processes of the invention.

公开(公告)号：US09938552B2

公开(公告)日：2018-04-10

申请号：US15026486

申请日：2014-10-31

Applicant: Novozymes A/S

Inventor： Hui Xu ,  Jesper Frickman ,  Jiyin Liu ,  Katja Salomon Johansen ,  Hong Zhi Huang

IPC: C12N9/42 ,  C12P19/14 ,  C12P19/02 ,  C12P7/10

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P2203/00 ,  Y02E50/16

Abstract: The invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a catalase and a cellulolytic enzyme composition comprising a GH61 polypeptide in the presence of dissolved oxygen at a concentration of greater than 10% of the saturation level. The invention also related to methods of producing desired fermentation products, such as ethanol, using a method including a saccharification step of the invention.

公开(公告)号：US20160298154A1

公开(公告)日：2016-10-13

申请号：US14916119

申请日：2014-09-04

Applicant: Novozymes A/S

Inventor： Jiyin Liu ,  Hui Xu ,  Feng Xu ,  Ye Chen ,  Terry Green

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12N9/0061 ,  C12N9/0065 ,  C12N9/0071 ,  C12P19/02 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y110/03002 ,  C12Y111/01006 ,  C12Y302/01004 ,  C13K1/02 ,  Y02E50/17

Abstract: The present invention relates to methods for increasing hydrolysis of a cellulosic material, comprising: hydrolyzing the cellulosic material with an enzyme composition in the presence of a combination of an AA9 polypeptide and one or more oxidoreductases selected from the group consisting of a catalase, a laccase, and a peroxidase.

Abstract translation: 本发明涉及增加纤维素材料水解的方法，其包括：在AA9多肽和一种或多种选自过氧化氢酶，漆酶的一种或多种氧化还原酶的组合存在下，用酶组合物水解纤维素材料 ，和过氧化物酶。

公开(公告)号：US10513721B2

公开(公告)日：2019-12-24

申请号：US15554384

申请日：2016-03-11

Applicant: Novozymes A/S

Inventor： Jiyin Liu ,  James Michael Broering ,  Keith McFarland ,  Xin Li

IPC: C12P19/14 ,  C12P7/06 ,  C12P19/02 ,  C12P7/10

Abstract: The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of one or more cellulolytic enzymes and at least one of an oxidoreductase and an AA9 polypeptide having cellulolytic enhancing activity is added, followed by a latter stage in which a second enzyme composition comprising one or more cellulases is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.

公开(公告)号：US20180273984A1

公开(公告)日：2018-09-27

申请号：US16006521

申请日：2018-06-12

Applicant: Novozymes A/S ,  Novozymes Inc. ,  Novozymes North America, Inc.

Inventor： Qiming Jin ,  Jiyin Liu ,  Bjorn Lennart Pierre Alexander Cassland ,  Donald L. Higgins

IPC: C12P7/10

CPC classification number: C12P7/10 ,  Y02E50/16

Abstract: The invention relates to a process of detoxifying pre-treated lignocellulose-containing material comprising subjecting the pre-treated lignocellulose-containing material to one or more phenolic compound oxidizing enzymes.

公开(公告)号：US20180044706A1

公开(公告)日：2018-02-15

申请号：US15554384

申请日：2016-03-11

Applicant: Novozymes A/S

Inventor： Jiyin Liu ,  James Michael Broering ,  Keith McFarland ,  Xin Li

IPC: C12P19/14 ,  C12P7/06

CPC classification number: C12P19/14 ,  C12P7/06 ,  C12P7/10 ,  C12P19/02 ,  Y02E50/16 ,  Y02E50/17

Abstract: The invention relates to processes of multi-stage hydrolysis where different enzyme compositions are added in at least two stages of hydrolysis. In a first stage, a first enzyme composition of one or more cellulolytic enzymes and at least one of an oxidoreductase and an AA9 polypeptide having cellulolytic enhancing activity is added, followed by a latter stage in which a second enzyme composition comprising one or more cellulases is added. Also provided are processes for obtaining hydrolysis products and fermentation products using processes of the invention.

公开(公告)号：US20160215314A1

公开(公告)日：2016-07-28

申请号：US15026486

申请日：2014-10-31

Applicant: NOVOZYMES A/S

Inventor： Hui Xu ,  Jesper Frickman ,  Jiyin Liu ,  Katja Salomon Johansen ,  Hong Zhi Huang

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12P19/14 ,  C12P7/10 ,  C12P19/02 ,  C12P2203/00 ,  Y02E50/16

Abstract: The invention relates to methods of saccharifying a cellulosic material comprising subjecting the cellulosic material to a catalase and a cellulolytic enzyme composition comprising a GH61 polypeptide in the presence of dissolved oxygen at a concentration of greater than 10% of the saturation level. The invention also related to methods of producing desired fermentation products, such as ethanol, using a method including a saccharification step of the invention.

Abstract translation: 本发明涉及将纤维素材料糖化的方法，包括在溶解氧存在下浓度大于饱和水平的10％的过氧化氢酶和含有GH61多肽的纤维素分解酶组合物。 本发明还涉及使用包括本发明的糖化步骤的方法生产所需发酵产物如乙醇的方法。

公开(公告)号：US20150140612A1

公开(公告)日：2015-05-21

申请号：US14413829

申请日：2013-07-18

Applicant: Novozymes A/S

Inventor： Ye Chen ,  Jiyin Liu ,  Terry Green ,  Mark V. Stevens ,  Hui Xu

IPC: C12P19/02 ,  C12P19/14

CPC classification number: C12P19/02 ,  C12N9/0065 ,  C12N9/2437 ,  C12P7/10 ,  C12P19/14 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/17

Abstract: The present invention relates to methods for increasing hydrolysis of a pretreated cellulosic material, comprising subjecting the pretreated cellulosic material to a cellulolytic enzyme composition; a polypeptide having cellulolytic enhancing activity; a Peroxidase; and a nonionic surfactant and/or cationic surfactant, at conditions suitable for hydrolyzing the pretreated lignocellulosic material. The invention also relates to processes for producing a fermentation product comprising a hydrolysis step of the invention and a composition suitable for use in a method of the invention.

Abstract translation: 本发明涉及增加预处理纤维素材料水解的方法，包括将经预处理的纤维素材料经受纤维素分解酶组合物; 具有纤维素分解增强活性的多肽; 过氧化物酶; 和非离子表面活性剂和/或阳离子表面活性剂，在适于水解预处理的木质纤维素材料的条件下。 本发明还涉及用于生产包含本发明的水解步骤和适用于本发明方法的组合物的发酵产物的方法。