公开(公告)号：US06451995B1

公开(公告)日：2002-09-17

申请号：US09142974

申请日：1998-09-18

申请人： Nai-Kong V. Cheung ,  Steven M. Larson ,  Hong-Fen Guo ,  Ken Rivlin ,  Michel Sadelain

发明人： Nai-Kong V. Cheung ,  Steven M. Larson ,  Hong-Fen Guo ,  Ken Rivlin ,  Michel Sadelain

IPC分类号： C12N1513

CPC分类号： C07K16/3084 ,  A61K47/6898 ,  A61K47/6899 ,  B82Y5/00 ,  C07K14/36 ,  C07K14/70517 ,  C07K16/30

摘要： Recombinant antibody constructs comprise the variable regions of the heavy and light chains of anti-GD2 antibodies. These antibody constructs may be coupled to a label such as a radiolabel or to a protein such as streptavidin or pro-drug converting enzymes for use in imaging or therapeutic applications. The antibody constructs may also be transduced into T cells to produce populations of T cells which target GD2-producing tumor cells.

摘要翻译： 重组抗体构建体包含抗GD2抗体的重链和轻链的可变区。 这些抗体构建体可以偶联到诸如放射性标记的标记物或蛋白质例如链霉亲和素或前药转化酶，用于成像或治疗应用。 抗体构建体也可以被转导入T细胞以产生靶向产生GD2的肿瘤细胞的T细胞群体。

公开(公告)号：US07666424B2

公开(公告)日：2010-02-23

申请号：US10273762

申请日：2002-10-17

申请人： Nai-Kong Cheung ,  Hong-Fen Guo

发明人： Nai-Kong Cheung ,  Hong-Fen Guo

IPC分类号： A61K39/00

CPC分类号： C07K16/4266 ,  A01K2217/05 ,  A61K47/6865 ,  A61K47/6873 ,  A61K49/0004 ,  A61K49/0006 ,  A61K51/1045 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/18 ,  C07K16/30 ,  C07K16/3053 ,  C07K16/3084 ,  C07K16/4208 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2317/77 ,  C07K2319/00

摘要： This invention provides a method for identifying cells expressing a target single chain antibody (scFv) directed against a target antigen from a collection of cells that includes cells that do not express the target scFv, comprising the step of combining the collection of cells with an anti-idiotype directed to an antibody specific for the target antigen and detecting interaction, if any, of the anti-idiotype with the cells, wherein the occurrence of an interaction identifies the cell as one which expresses the target scFv. This invention also provides a method for making a single chain antibody (scFv) directed against an antigen, wherein the selection of clones is made based upon interaction of those clones with an appropriate anti-idiotype, and heretofore inaccessible scFv so made. This invention provides the above methods or any combination thereof. Finally, this invention provides various uses of these methods.

摘要翻译： 本发明提供了从包含不表达靶标scFv的细胞的细胞集合中鉴定表达靶抗原的目标单链抗体（scFv）的细胞的方法，包括将细胞收集与抗 - 针对针对靶抗原特异性的抗体，并检测与细胞的抗独特型相互作用（如果有的话），其中相互作用的发生将细胞识别为表达靶scFv的细胞。 本发明还提供了制备针对抗原的单链抗体（scFv）的方法，其中克隆的选择基于那些克隆与适当的抗独特型的相互作用，以及如此制备的迄今为止不可接近的scFv。 本发明提供了上述方法或其任何组合。 最后，本发明提供了这些方法的各种用途。