公开(公告)号：US10017774B2

公开(公告)日：2018-07-10

申请号：US13497729

申请日：2010-09-15

申请人： Jian Ye ,  Nam-Hai Chua ,  Jing Qu ,  Shi-Qiang Gao

发明人： Jian Ye ,  Nam-Hai Chua ,  Jing Qu ,  Shi-Qiang Gao

IPC分类号： C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.

公开(公告)号：US08921655B2

公开(公告)日：2014-12-30

申请号：US13141752

申请日：2009-12-16

申请人： Jian Ye ,  Nam Hai Chua ,  Jing Qu

发明人： Jian Ye ,  Nam Hai Chua ,  Jing Qu

IPC分类号： C12N15/11 ,  C07H21/04 ,  C12N15/113 ,  C12N15/63 ,  C12N15/82 ,  C12N15/83 ,  C12N15/84 ,  A01H5/00

CPC分类号： C12N15/8218

摘要： The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).

摘要翻译： 本发明涉及基因组尺度上的麻疯树基因的功能分析领域。 更具体地，本发明涉及使用病毒诱导的基因沉默在基因组规模上高麻醉麻疯树基因的高通量功能分析的方法。 该方法涉及使用烟草拨浪鼓病毒（TRV）。

公开(公告)号：US08853493B2

公开(公告)日：2014-10-07

申请号：US13376902

申请日：2010-06-10

申请人： Jian Ye ,  Nam Hai Chua ,  Jing Qu ,  Yun Feng Geng ,  Yun Ping Bu

发明人： Jian Ye ,  Nam Hai Chua ,  Jing Qu ,  Yun Feng Geng ,  Yun Ping Bu

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

摘要： The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.

摘要翻译： 本发明涉及使用病毒诱导基因沉默（VIGS）方法对棉花中基因的功能分析。 更具体地说，这种方法在烟草叮咬病毒（TRV）载体RNA1和RNA2的帮助下诱导棉花中的基因沉默，并且可以分析对棉花植物的表型效应。此外，本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因 在棉花植物和植物组织中受到强亚基因子启动子的影响。

公开(公告)号：US10100321B2

公开(公告)日：2018-10-16

申请号：US14395233

申请日：2012-04-19

申请人： Nam-Hai Chua ,  Jian Ye ,  Jing Qu

发明人： Nam-Hai Chua ,  Jian Ye ,  Jing Qu

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.

公开(公告)号：US20150074853A1

公开(公告)日：2015-03-12

申请号：US14395233

申请日：2012-04-19

申请人： Nam-Hai Chua ,  Jian Ye ,  Jing Qu

发明人： Nam-Hai Chua ,  Jian Ye ,  Jing Qu

IPC分类号： C12N15/82

CPC分类号： C12N15/8218 ,  C07K14/415 ,  C12N15/8234 ,  C12N15/8247 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.

摘要翻译： 本发明涉及植物分子生物学领域，尤其涉及棉花WRINKLED1样（WRINKLED 1-like，WREL）基因。 更具体地说，本发明涉及棉花WRIL基因，其产品作为参与脂肪酸生物合成的基因的转录因子。 本发明还涉及增加棉花纤维长度的方法。 在一个实施方案中，所述方法包括调节参与宿主棉花细胞中脂肪酸生物合成的酶的活性水平和/或培养宿主棉花细胞。 在另一个实施方案中，所述方法包括操纵能够调节参与脂肪酸生物合成的酶的转录因子。

公开(公告)号：US20120240288A1

公开(公告)日：2012-09-20

申请号：US13497729

申请日：2010-09-15

申请人： Jian Ye ,  Nam-Hai Chua ,  Jing Qu ,  Shi-Qiang Gao

发明人： Jian Ye ,  Nam-Hai Chua ,  Jing Qu ,  Shi-Qiang Gao

IPC分类号： A01H1/06 ,  A61K49/00

CPC分类号： C12N15/8218 ,  C12N15/8285 ,  C12N15/8286 ,  Y02A40/162 ,  Y02A40/164

摘要： The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.

摘要翻译： 本发明涉及使用病毒来在宿主中表达有害生物基因的昆虫等害虫防治领域。 更具体地说，本发明涉及一种用于快速筛选有害生物基因的方法，当害虫摄入表达病毒连接的害虫基因序列的宿主组织时，可导致害虫死亡。 本发明还涉及通过靶病毒序列的病毒表达来控制害虫的方法，以修饰害虫的细胞或组织中害虫基因的内源性表达。

公开(公告)号：US20120096595A1

公开(公告)日：2012-04-19

申请号：US13376902

申请日：2010-06-10

申请人： Jian Ye ,  Nam Hai Chua ,  Jing Qu ,  Yun Feng Geng ,  Yun Ping Bu

发明人： Jian Ye ,  Nam Hai Chua ,  Jing Qu ,  Yun Feng Geng ,  Yun Ping Bu

IPC分类号： A01H1/06 ,  C12Q1/68 ,  C12N15/82 ,  G01N33/53

CPC分类号： C12N15/8218 ,  C12N15/8261 ,  Y02A40/146

摘要： The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.

摘要翻译： 本发明涉及使用病毒诱导基因沉默（VIGS）方法对棉花中基因的功能分析。 更具体地说，这种方法在烟草叮咬病毒（TRV）载体RNA1和RNA2的帮助下诱导棉花中的基因沉默，并且可以分析对棉花植物的表型效应。此外，本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因 在棉花植物和植物组织中受到强亚基因子启动子的影响。

公开(公告)号：US20140096288A1

公开(公告)日：2014-04-03

申请号：US14118767

申请日：2011-09-28

申请人： Jian Ye ,  Jing Qu ,  Hui Zhu Mao

发明人： Jian Ye ,  Jing Qu ,  Hui Zhu Mao

IPC分类号： C12N15/82

CPC分类号： C12N15/8247 ,  C12N5/14 ,  C12N9/001 ,  C12N9/0083 ,  C12N15/8218 ,  C12P7/64 ,  C12Y103/01035 ,  Y02P20/52

摘要： The present invention relates to the field of plant molecular biology, more particularly Jatropha microsomal co6 oleate desaturases. The present invention also relates to Jatropha plants or plants of other oil crops having seeds with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention relates to Jatropha plants or plants of other oil crops where the plants exhibit elevated levels of oleic acid.

摘要翻译： 本发明涉及植物分子生物学领域，尤其涉及麻风树微粒体co6油酸酯去饱和酶。 本发明还涉及麻风树植物或其他油料作物的植物，其具有改变比例的单饱和多不饱和脂肪的种子。 特别地，本发明涉及麻风树植物或其他油料作物的植物，其中植物表现出高水平的油酸。

公开(公告)号：US09574205B2

公开(公告)日：2017-02-21

申请号：US14118767

申请日：2011-09-28

申请人： Jian Ye ,  Jing Qu ,  Hui Zhu Mao

发明人： Jian Ye ,  Jing Qu ,  Hui Zhu Mao

IPC分类号： C12N15/82 ,  C12N5/14 ,  C12N9/02 ,  C12P7/64

CPC分类号： C12N15/8247 ,  C12N5/14 ,  C12N9/001 ,  C12N9/0083 ,  C12N15/8218 ,  C12P7/64 ,  C12Y103/01035 ,  Y02P20/52

摘要： The present invention relates to the field of plant molecular biology, more particularly Jatropha microsomal co6 oleate desaturases. The present invention also relates to Jatropha plants or plants of other oil crops having seeds with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention relates to Jatropha plants or plants of other oil crops where the plants exhibit elevated levels of oleic acid.

摘要翻译： 本发明涉及植物分子生物学领域，尤其涉及麻风树微粒体co6油酸酯去饱和酶。 本发明还涉及麻风树植物或其他油料作物的植物，其具有改变比例的单饱和多不饱和脂肪的种子。 特别地，本发明涉及麻风树植物或其他油料作物的植物，其中植物表现出高水平的油酸。

公开(公告)号：US20120225666A1

公开(公告)日：2012-09-06

申请号：US13466628

申请日：2012-05-08

申请人： Jing Qu ,  Stephanie Teng-Ossman

发明人： Jing Qu ,  Stephanie Teng-Ossman

IPC分类号： H04W24/00

CPC分类号： H04W4/20 ,  H04W4/02 ,  H04W4/50 ,  H04W8/26

摘要： Disclosed is a method and system for use of a trusted server such as an over-the-air provisioning server to facilitate mobile location determination. If a mobile location server (MLS) sends a location-determination message to a mobile station and does not receive a response back from the mobile station, there is a chance that the mobile station is not provisioned with a correct address of the MLS and that the mobile station therefore did not respond. To help jump start the location-determination process, the MLS may respond to that situation by instead causing a trusted server such as an over-the-air-service-provisioning (OTASP) server to send a location-determination message to the mobile station, triggering a response from the mobile station.

摘要翻译： 公开了一种使用诸如空中供应服务器之类的信任服务器来促进移动位置确定的方法和系统。 如果移动定位服务器（MLS）向移动台发送位置确定消息并且没有从移动台接收到响应，那么移动台有机会没有为MLS提供正确的地址， 所以移动台没有回应。 为了帮助启动位置确定过程，MLS可以通过相反地引起诸如空中服务供应（OTASP）服务器的可信服务器来向移动台发送位置确定消息来响应该情况 触发来自移动台的响应。