公开(公告)号：US4745069A

公开(公告)日：1988-05-17

申请号：US586581

申请日：1984-03-06

申请人： Nancy G. Mayne ,  J. Paul Burnett ,  Ramamoorthy Belegaje ,  Hansen M. Hsiung

发明人： Nancy G. Mayne ,  J. Paul Burnett ,  Ramamoorthy Belegaje ,  Hansen M. Hsiung

IPC分类号： C12N15/66 ,  C12N15/70 ,  C12N15/00 ,  C07H21/02 ,  C12N1/20 ,  C12P19/34 ,  C12P21/00

CPC分类号： C12N15/66 ,  C12N15/70 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  Y10S930/12

摘要： A recombinant DNA cloning vector useful for expressing exogenous protein is described, which comprises(a) a DNA segment containing a functional origin of replication;(b) one or more DNA segments, each of which conveys to a transformable host cell a property useful for selection when said vector is transformed into said host cell; and(c) a DNA segment comprising a sequence that defines, in tandem,(1) the promoter of a lipoprotein expression control sequence,(2) the 5' untranslated region of a lipoprotein expression control sequence and(3) a translation start codon followed, without interposition of a portion or all of a nucleotide sequence coding for endogenous protein, by a nucleotide sequence coding for an enterokinase cleavage site to which is joined, without interruption, a nucleotide sequence coding for an exogenous protein.

摘要翻译： 描述了可用于表达外源蛋白质的重组DNA克隆载体，其包含（a）含有功能复制起点的DNA片段; （b）一个或多个DNA片段，当将所述载体转化到所述宿主细胞中时，每个DNA片段向可转化宿主细胞传送可用于选择的性质; （c）包含一系列序列的DNA片段，其一致地限定（1）脂蛋白表达控制序列的启动子，（2）脂蛋白表达控制序列的5'非翻译区和（3）翻译起始密码子 随后，通过编码与外源蛋白质编码的核苷酸序列不连续地连接的肠激酶切割位点的核苷酸序列，不插入编码内源蛋白质的一部分或全部核苷酸序列。