公开(公告)号：US20160281057A1

公开(公告)日：2016-09-29

申请号：US15032446

申请日：2014-10-30

申请人： Naoki YAMASHITA ,  TOKYO METROPOLITAN GERIATRIC HOSPITAL AND INSTITUTE OF GERONTOLOGY

发明人： Naoki Yamashita ,  Kumiko Nakata ,  Ikuroh Ohsawa

IPC分类号： C12N5/076

CPC分类号： C12N5/061 ,  A61K33/00 ,  C12N2500/02

摘要： The present invention addresses the problem of providing an agent for improving sperm-motility, in particular, an agent for improving forward-sperm-motility, for use on reduced-motility sperm in male infertility treatment. Prepared is a gaseous agent for improving sperm-motility consisting of a gas that contains hydrogen molecules in the amount of 1% (v/v) or higher, for example, a gas that contains hydrogen molecules in an amount of between 45 and 55% (v/v), or a liquid agent for improving sperm-motility consisting of a liquid such as physiological saline, a culture solution, or a buffer solution containing hydrogen molecules in the amount of 1% or higher of the saturation solubility thereof, for example, a liquid in which hydrogen molecules have been dissolved by bubbling. The gaseous agent for improving sperm-motility can improve the motility of sperm by contacting hydrogen gas and either semen or a dilution thereof with each other in a gaseous phase. The liquid agent for improving sperm-motility can improve the motility of sperm when used as a washing solution or diluent for semen.

摘要翻译： 本发明解决了在男性不孕症治疗中提供用于改善精子运动性的药剂，特别是用于改善前精子运动性的药剂用于减少精子活力的问题。 制备的是用于提高精子活力的气态剂，其由含有1％（v / v）以上的氢分子的气体组成，例如含有45〜55％的氢分子的气体， （v / v）或用于提高精液运动性的液体剂，其由例如生理盐水，培养溶液或含有其饱和溶解度的1％以上的氢分子的缓冲溶液组成的液体试剂用于 例如，通过鼓泡将氢分子溶解的液体。 用于提高精子活力的气态剂可以通过在气相中将氢气和精液或其稀释液彼此接触来改善精子的运动性。 用于提高精子活力的液体剂可以提高精子在作为精液的洗涤液或稀释剂时的运动性。

公开(公告)号：US10160949B2

公开(公告)日：2018-12-25

申请号：US15032446

申请日：2014-10-30

申请人： Naoki Yamashita ,  Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology

发明人： Naoki Yamashita ,  Kumiko Nakata ,  Ikuroh Ohsawa

IPC分类号： C12N5/076 ,  A61K33/00

摘要： The present invention addresses the problem of providing an agent for improving sperm-motility, in particular, an agent for improving forward-sperm-motility, for use on reduced-motility sperm in male infertility treatment. Prepared is a gaseous agent for improving sperm-motility consisting of a gas that contains hydrogen molecules in the amount of 1% (v/v) or higher, for example, a gas that contains hydrogen molecules in an amount of between 45 and 55% (v/v), or a liquid agent for improving sperm-motility consisting of a liquid such as physiological saline, a culture solution, or a buffer solution containing hydrogen molecules in the amount of 1% or higher of the saturation solubility thereof, for example, a liquid in which hydrogen molecules have been dissolved by bubbling.

公开(公告)号：US20140249363A1

公开(公告)日：2014-09-04

申请号：US14041338

申请日：2013-09-30

申请人： Naoki Yamashita

发明人： Naoki Yamashita ,  Kumiko Nakata

IPC分类号： G01N33/50 ,  A61B17/435

CPC分类号： G01N33/9433 ,  A61B17/435 ,  G01N2800/367 ,  G01N2800/52

摘要： The invention provides a new method for evaluating transfer embryos including blastocysts used for in vitro fertilization in fertility treatment, and a method for evaluating transfer embryos using a new biomarker necessary for evaluation. The method comprises the steps of (a) providing a test object, for example, a culture solution of a human blastocyst, containing norepinephrine (noradrenaline) released from a transfer embryo, such as a human blastocyst, obtained from a subject; (b) quantitatively analyzing norepinephrine in the test object by a combination of ultra high performance liquid chromatography and mass spectrometry or the like; (c) predicting the quality of the transfer embryo based on the amount of norepinephrine from analysis results obtained; and (d) transferring the embryo into a suitable female recipient for implantation, if the transfer embryo is predicted to be of good quality and/or to lead to the establishment of a viable pregnancy based on step (c).

摘要翻译： 本发明提供了一种用于评估转移胚胎的新方法，包括用于生育治疗中的体外受精的囊胚，以及使用评估所需的新生物标志物来评估转移胚胎的方法。 该方法包括以下步骤：（a）提供测试对象，例如从受试者获得的转移胚（例如人类囊胚）中释放的含有去甲肾上腺素（去甲肾上腺素）的人类胚泡培养液; （b）通过超高效液相色谱和质谱等的组合定量分析试验对象中的去甲肾上腺素; （c）根据获得的分析结果预测去甲肾上腺素的转移胚胎质量; 如果根据步骤（c）预测转移胚胎具有良好的质量和/或导致建立可行的怀孕，则将胚胎转移到合适的雌性受体中以进行植入。

公开(公告)号：US09354244B2

公开(公告)日：2016-05-31

申请号：US14041338

申请日：2013-09-30

申请人： Naoki Yamashita

发明人： Naoki Yamashita ,  Kumiko Nakata

IPC分类号： A61B17/435 ,  G01N33/94

CPC分类号： G01N33/9433 ,  A61B17/435 ,  G01N2800/367 ,  G01N2800/52

摘要： The invention provides a new method for evaluating transfer embryos including blastocysts used for in vitro fertilization in fertility treatment, and a method for evaluating transfer embryos using a new biomarker necessary for evaluation. The method comprises the steps of (a) providing a test object, for example, a culture solution of a human blastocyst, containing norepinephrine (noradrenaline) released from a transfer embryo, such as a human blastocyst, obtained from a subject; (b) quantitatively analyzing norepinephrine in the test object by a combination of ultra high performance liquid chromatography and mass spectrometry or the like; (c) predicting the quality of the transfer embryo based on the amount of norepinephrine from analysis results obtained; and (d) transferring the embryo into a suitable female recipient for implantation, if the transfer embryo is predicted to be of good quality and/or to lead to the establishment of a viable pregnancy based on step (c).

摘要翻译： 本发明提供了一种用于评估转移胚胎的新方法，包括用于生育治疗中的体外受精的囊胚，以及使用评估所需的新生物标志物来评估转移胚胎的方法。 该方法包括以下步骤：（a）提供测试对象，例如从受试者获得的转移胚（例如人类囊胚）中释放的含有去甲肾上腺素（去甲肾上腺素）的人类胚泡培养液; （b）通过超高效液相色谱和质谱等的组合定量分析试验对象中的去甲肾上腺素; （c）根据获得的分析结果预测去甲肾上腺素的转移胚胎质量; 如果根据步骤（c）预测转移胚胎具有良好的质量和/或导致建立可行的怀孕，则将胚胎转移到合适的雌性受体中以进行植入。