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公开(公告)号：US07514216B2
公开(公告)日：2009-04-07
申请号：US11188743
申请日：2005-07-26
IPC分类号： C12Q1/68
CPC分类号： C12N15/81
摘要： Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.
摘要翻译： 酵母细胞被诱变以获得所需的突变体。 诱变由缺陷错配修复系统介导，可以使用常规的外源应用诱变剂进行增强。 具有缺陷错配修复系统的酵母细胞是可超过可变的，但是在选择所需的突变酵母菌株之后，可以通过将错配修复系统恢复到适当的功能来使它们变得遗传稳定。
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公开(公告)号：US20090137427A1
公开(公告)日：2009-05-28
申请号：US11976019
申请日：2007-10-19
CPC分类号： C12N15/1024 , C07K14/47 , C12N15/01 , C12N15/102
摘要： Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. The enhanced rate of mutation can be further augmented using mutagens. Moreover, the hypermutability of mismatch repair deficient cells can be remedied to stabilize cells or mammals with useful mutations.
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公开(公告)号：US20080318321A1
公开(公告)日：2008-12-25
申请号：US11930391
申请日：2007-10-31
IPC分类号： C12N15/87
CPC分类号： C12N15/1024 , C12N15/01 , C12N15/102
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.
摘要翻译： 操纵细菌以使用错配修复蛋白质的显性负等位基因产生理想的输出性状。 通过错配修复缺陷和外源应用诱变剂的组合实现增强的超突变。 通过恢复对细菌的错配修复活性获得含有所需输出性状的稳定细菌。
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公开(公告)号：US07026119B2
公开(公告)日：2006-04-11
申请号：US09780675
申请日：2001-02-12
CPC分类号： C12N15/1024 , C12N15/01 , C12N15/102
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.
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公开(公告)号：US07759121B2
公开(公告)日：2010-07-20
申请号：US11930400
申请日：2007-10-31
IPC分类号： C12N15/01
CPC分类号： C12N15/81
摘要： Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be rendered genetically stable by restoring the mismatch repair system to proper functionality.
摘要翻译： 酵母细胞被诱变以获得所需的突变体。 诱变由缺陷错配修复系统介导，可以使用常规的外源应用诱变剂进行增强。 具有缺陷错配修复系统的酵母细胞是可超过可变的，但是在选择所需的突变酵母菌株之后，可以通过将错配修复系统恢复到适当的功能来使它们变得遗传稳定。
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公开(公告)号：US07704689B2
公开(公告)日：2010-04-27
申请号：US11128420
申请日：2005-05-13
IPC分类号： C12Q1/68
CPC分类号： C12N15/1024 , C07K14/415 , C12N15/8216 , C12N15/8241
摘要： Blockade of mismatch repair in a plant can lead to hypermutation and a new genotype and/or phenotype. One approach used to generate hypermutable plants is through the expression of dominant negative alleles of mismatch repair genes in transgenic plants or derived cells. By introducing these genes into cells and transgenic plants, new cell lines and plant varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. Moreover, methods to inhibit the expression and activity of endogenous plant MMR genes and their encoded products are also useful to generate hypermutable plants.
摘要翻译： 植物中错配修复的阻断可导致超突变和新的基因型和/或表型。 用于产生超可变植物的一种方法是通过在转基因植物或衍生细胞中表达错配修复基因的显性负等位基因。 通过将这些基因引入细胞和转基因植物，可以比通过依赖于突变的自然速率更有效地制备具有新颖和有用性质的新细胞系和植物品种。 此外，抑制内源植物MMR基因及其编码产物的表达和活性的方法也可用于产生高可变性植物。
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公开(公告)号：US06656736B2
公开(公告)日：2003-12-02
申请号：US09788657
申请日：2001-02-21
IPC分类号： C12N1574
CPC分类号： C12N15/81
摘要： Yeast cells are mutagenized to obtain desirable mutants. Mutagenesis is mediated by a defective mismatch repair system which can be enhanced using conventional exogenously applied mutagens. Yeast cells with the defective mismatch repair system are hypermutable, but after selection of desired mutant yeast strains, they can be be rendered genetically stable by restoring the mismatch repair system to proper functionality.
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公开(公告)号：US06825038B2
公开(公告)日：2004-11-30
申请号：US09853646
申请日：2001-05-14
IPC分类号： C12N1500
CPC分类号： C12N15/1024 , C07K14/47 , C12N15/01 , C12N15/102
摘要： Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. By introducing these genes into cells and transgenic animals, new cell lines and animal varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. The enhanced rate of mutation can be further augmented using mutagens. Moreover, the hypermutability of mismatch repair deficient cells can be remedied to stabilize cells or mammals with useful mutations.
摘要翻译： 人类错配修复基因的主要阴性等位基因可用于产生超可变细胞和生物体。 通过将这些基因引入细胞和转基因动物，可以比通过依赖于突变的自然速率更有效地制备具有新颖和有用性质的新细胞系和动物品种。 使用诱变剂可以进一步增强突变率的增强。 此外，错配修复缺陷细胞的高度易失性可以补救以稳定具有有用突变的细胞或哺乳动物。
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公开(公告)号：US07892832B2
公开(公告)日：2011-02-22
申请号：US11930391
申请日：2007-10-31
CPC分类号： C12N15/1024 , C12N15/01 , C12N15/102
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.
摘要翻译： 操纵细菌以使用错配修复蛋白质的显性负等位基因产生理想的输出性状。 通过错配修复缺陷和外源应用诱变剂的组合实现增强的超突变。 通过恢复对细菌的错配修复活性获得含有所需输出性状的稳定细菌。
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公开(公告)号：US07695969B2
公开(公告)日：2010-04-13
申请号：US11360995
申请日：2006-02-24
CPC分类号： C12N15/1024 , C12N15/01 , C12N15/102
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.
摘要翻译： 操纵细菌以使用错配修复蛋白质的显性负等位基因产生理想的输出性状。 通过错配修复缺陷和外源应用诱变剂的组合实现增强的超突变。 通过恢复对细菌的错配修复活性获得含有所需输出性状的稳定细菌。
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