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公开(公告)号:US5674678A
公开(公告)日:1997-10-07
申请号:US205603
申请日:1988-06-02
CPC分类号: C12P21/02 , C12N1/38 , C12N15/70 , C12N9/2471 , C12Y302/01023 , C07K2319/00 , C07K2319/02
摘要: Expression of a desired gene and production of a product from the desired gene can be obtained effectively by cultivating a microorganism containing a hybrid plasmid comprising the desired gene, a vector and a promoter in its cell, and adding an inducer and a nutriment simultaneously to the culture medium at the time when the nutriment originally present in the culture medium is almost consumed.
摘要翻译: 可以通过在其细胞中培养含有所需基因,载体和启动子的杂交质粒的微生物,同时向其中添加诱导剂和营养物,从而有效地获得所需基因的表达和产生所需基因的产物 培养基中最初存在于培养基中的营养物几乎被消耗时的培养基。