摘要:
Expression of a desired gene and production of a product from the desired gene can be obtained effectively by cultivating a microorganism containing a hybrid plasmid comprising the desired gene, a vector and a promoter in its cell, and adding an inducer and a nutriment simultaneously to the culture medium at the time when the nutriment originally present in the culture medium is almost consumed.
摘要:
By culturing cells in an apparatus containing a first zone in which a cell suspended medium is present and a second zone in which liquid containing a physiological saline solution and/or nutrient sources is present, the first zone being separated from the second zone by a septum with characteristics, such as pores size or electrostatic charges, which impedes the cells from freely permeating through, while allowing at least one of nutrient sources, cell wastes or the product to move between the first zone and the second zone via the septum to proliferate cells in the first zone, wherein a flow rate via the septum is controlled in such a manner that the cell count moved from the first zone into the second zone via the septum does not exceed the cell count increased by cell growth in the first zone. Thus, cell culture at a high cell density can be achieved in a large scale.
摘要:
The invention relates to an improvement of the floc-formation property of activated sludge contained in waste water.A waste water treatment process comprises steps culturing a novel strain-Alcaligenes faecalis HRL-1 - and adding the cultured cells to to-be-treated waste water.
摘要:
The invention relates to an improvement of the floc-formation property of activated sludge contained in waste water.A waste water treatment process comprises steps culturing a novel strain--Alcaligenes faecalis HRL--1--and adding the cultured cells to to-be-treated waste water.
摘要:
Cultivation of microorganisms or animal cells or plant cells is carried out to produce high density cultivation, high cell yield and high production of desired metabolite products by monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cells or plant cells to control the acetate concentration to a set value or less. Preferably, an acetic acid-producing bacterium that is inhibited by acetic acid and is capable of assimilating acetic acid is cultured to produce biologically active substances such as enzymes. The bacterium may be a recombinant Escherichia coli and an inducer which acts on a promoter in an expression vector is added to produce the desired metabolite product. The inducer is 3-.beta.- indolylacrylic acid when the promoter is trp-promoter or isopropyl-.beta.-D-thiogalactoside when the promoter is lac-promoter or tacpromoter.
摘要:
A method of controlling culture wherein cells are aerobically cultured to produce metabolite is characterized by adding substrate and/or inducer to the culture broth, judging the endpoint of the growth of the cells and/or the production of the metabolite or terminating the culture under the guidance of a change in the ratio of the first parameter based on the amount of carbon dioxide evolved by the cell culture to the second parameter based on the amount of oxygen consumed by the cell culture. The ratio is a respiratory quotient, for instance.
摘要:
This invention relates to an efficient process for culturing recombinant cells which comprises a step of removing a cell proliferation inhibiting substance and/or a substance which inhibits induction of the desired product and a step of inducing production of the desired product.
摘要:
The radius of curvature of the outer surface of a panel is 10,000 mm or more, and a shadow mask is made of a material containing 95% or more of iron. A sagging amount change curve along a curve C1 on the surface of the shadow mask, which a plane passing through a center P0 of a useful area of the shadow mask and parallel to a tube axis and a major axis crosses, satisfies a particular Condition 1. Assuming that an intersection between the curve C1 and a useful area end of the shadow mask is a major axis end PL, a distance from the center P0 to the major axis end PL along a major axis is W, and a point on the curve C1 away from the center P0 by ⅔×W in the major axis direction is P1, a sagging amount change curve along a curve C2 on the surface of the shadow mask, which a plane passing through the point P1 and parallel to the tube axis and the minor axis crosses, satisfies a particular Condition 2. Consequently, a color picture tube can be realized, which has satisfactory visibility, and less degradation in color purity caused by doming while having a shadow mask made of an inexpensive material with satisfactory moldability.
摘要:
The radius of curvature of the outer surface of a panel is 10,000 mm or more, and a shadow mask is made of a material containing 95% or more of iron. A sagging amount change curve along a curve C1 on the surface of the shadow mask, which a plane passing through a center P0 of a useful area of the shadow mask and parallel to a tube axis and a major axis crosses, satisfies a particular Condition 1. Assuming that an intersection between the curve C1 and a useful area end of the shadow mask is a major axis end PL, a distance from the center P0 to the major axis end PL along a major axis is W, and a point on the curve C1 away from the center P0 by ⅔×W in the major axis direction is P1, a sagging amount change curve along a curve C2 on the surface of the shadow mask, which a plane passing through the point P1 and parallel to the tube axis and the minor axis crosses, satisfies a particular Condition 2. Consequently, a color picture tube can be realized, which has satisfactory visibility, and less degradation in color purity caused by doming while having a shadow mask made of an inexpensive material with satisfactory moldability.
摘要:
The mask body satisfies the following conditions, (ZMD−ZMH)/LMS≧0.020, and 0.065≦ZMD/LMD≦0.095 where in a substantially rectangular effective region with a predetermined curvature of the mask body, LMD is a distance between a central part and a diagonal-axis end of the effective region, LMS is a distance between a horizontal-axis end and the diagonal-axis end of the effective region, ZMD is a height difference between the central part and the diagonal-axis end in a direction of the tube axis, and ZMH is a height difference between the central part and the horizontal-axis end in the direction of the tube axis. With this structure, the mechanical strength of the mask body can be improved, and deformation of the mask body in a fabrication process and due to external shock can be prevented.