公开(公告)号：US10457749B2

公开(公告)日：2019-10-29

申请号：US15068916

申请日：2016-03-14

申请人： NovImmune SA

发明人： Nicolas Fouque ,  Jean François Depoisier ,  Keith Wilson ,  Judith Vajda ,  Egbert Müller ,  Romain Dabre

IPC分类号： C07K16/46 ,  C07K1/36 ,  C07K1/16 ,  C07K1/22 ,  C07K1/20 ,  C07K16/00 ,  C07K16/24 ,  C07K16/28

摘要： The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

公开(公告)号：US20160264685A1

公开(公告)日：2016-09-15

申请号：US15068916

申请日：2016-03-14

申请人： NovImmune SA

发明人： Nicolas Fouque ,  Jean François Depoisier ,  Keith Wilson ,  Judith Vajda ,  Egbert Müller ,  Romain Dabre

IPC分类号： C07K16/46 ,  C07K1/36 ,  C07K1/22 ,  C07K1/16 ,  C07K1/20

CPC分类号： C07K16/468 ,  C07K1/16 ,  C07K1/165 ,  C07K1/20 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00 ,  C07K16/248 ,  C07K16/249 ,  C07K16/2866 ,  C07K2317/31 ,  C07K2317/32 ,  C07K2317/52

摘要： The invention relates to the purification of bispecific antibodies carrying a different specificity for each binding site of the immunoglobulin molecule from a mixture of monospecific antibodies. The bispecific antibodies are composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain. This invention in particular relates to the isolation of these bispecific antibodies from mixtures that contain monospecific antibodies having two Kappa light chains or portions thereof and monospecific antibodies having two Lambda light chains or portions thereof. The invention also provides the methods of efficiently purifying these bispecific antibodies.

摘要翻译： 本发明涉及从单特异性抗体的混合物纯化对免疫球蛋白分子的每个结合位点具有不同特异性的双特异性抗体。 双特异性抗体由单个重链和两个不同的轻链组成，一个含有κ恒定结构域，另一个是λ恒定结构域。 本发明特别涉及从包含具有两个κ轻链或其部分的单特异性抗体和具有两个λ轻链或其部分的单特异性抗体的混合物中分离出这些双特异性抗体。 本发明还提供了有效纯化这些双特异性抗体的方法。