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Patent Agency Ranking
公开(公告)号：US08999685B2
公开(公告)日：2015-04-07
申请号：US14269591
申请日：2014-05-05
Applicant: Novozymes, Inc.
Inventor： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
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公开(公告)号：US20140234925A1
公开(公告)日：2014-08-21
申请号：US14269591
申请日：2014-05-05
Applicant: Novozymes, Inc.
Inventor： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
IPC: C12P7/46
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。
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公开(公告)号：US20150024446A1
公开(公告)日：2015-01-22
申请号：US14510582
申请日：2014-10-09
Applicant: Novozymes, Inc.
Inventor： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
Abstract translation: 本发明涉及生产C4二羧酸的方法，其包括：（a）培养丝状真菌宿主细胞，所述丝状真菌宿主细胞包含选自以下的多核苷酸：编码C4二羧酸转运蛋白的异源第一多核苷酸，编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中当在相同条件下培养时，丝状真菌宿主细胞能够分泌与不含异源多核苷酸的丝状真菌宿主细胞相比增加的C4二羧酸水平; 和（b）回收C4二羧酸。 本发明还涉及增加C4二羧酸生产，丝状真菌宿主细胞和苹果酸脱氢酶变体的方法。
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公开(公告)号：US09447440B2
公开(公告)日：2016-09-20
申请号：US14159786
申请日：2014-01-21
Applicant: Novozymes, Inc.
Inventor： Amanda Fischer , Stephen Brown , Sheryl Luttringer
Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
Abstract translation: 本发明涉及具有碳酸氢根转运蛋白活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。
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公开(公告)号：US20140134688A1
公开(公告)日：2014-05-15
申请号：US14159786
申请日：2014-01-21
Applicant: Novozymes, Inc.
Inventor： Amanda Fischer , Stephen Brown , Sheryl Luttringer
IPC: C12P7/46
Abstract: The present invention relates to isolated polypeptides having bicarbonate transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid.
Abstract translation: 本发明涉及具有碳酸氢根转运蛋白活性的分离多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法，以及生产C4-二羧酸如苹果酸的方法。
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