公开(公告)号：US20220186263A1

公开(公告)日：2022-06-16

申请号：US17601303

申请日：2020-04-03

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Yoshihiro UNO ,  Yuko KOTANI ,  Yoshiki MIYASAKA ,  Yuichiro OKA ,  Makoto SATO

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10

Abstract: The present inventors have found that the use of a site-specific nuclease system which includes a combination of a molecule that simultaneously targets and cleaves one homology arm sequence of the donor DNA and the genomic sequence corresponding to the homology arm sequence, and a molecule that targets and cleaves the genomic region in the vicinity of the cleavage site by that molecule causes repair between genomic DNA and donor DNA through both non-homologous end joining and homologous recombination, making it possible to produce cells and organisms with knocked-in long donor sequences with high efficiency and accuracy.

公开(公告)号：US20200029538A1

公开(公告)日：2020-01-30

申请号：US16463883

申请日：2017-11-24

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Yoshiki MIYASAKA

IPC: A01K67/027 ,  C12N15/90 ,  C12N15/10

Abstract: A method for producing a genome edited cell or a non-human organism, comprising the step of introducing (a) an artificial nuclease system which cleaves both ends of a genome editing target region, and (b) a nucleic acid sequence formed by arranging a 5′-side homology arm sequence, a donor DNA sequence, and a 3′-side homology arm sequence in this order from a 5′-side, the 5′-side homology arm sequence being a homologous sequence of one nucleic acid sequence outside the genome editing target region, and the 3′-side homology arm sequence being a homologous sequence of the other nucleic acid sequence outside the genome editing target region, into a cell or a non-human organism.