公开(公告)号：US20220186263A1

公开(公告)日：2022-06-16

申请号：US17601303

申请日：2020-04-03

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Yoshihiro UNO ,  Yuko KOTANI ,  Yoshiki MIYASAKA ,  Yuichiro OKA ,  Makoto SATO

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10

Abstract: The present inventors have found that the use of a site-specific nuclease system which includes a combination of a molecule that simultaneously targets and cleaves one homology arm sequence of the donor DNA and the genomic sequence corresponding to the homology arm sequence, and a molecule that targets and cleaves the genomic region in the vicinity of the cleavage site by that molecule causes repair between genomic DNA and donor DNA through both non-homologous end joining and homologous recombination, making it possible to produce cells and organisms with knocked-in long donor sequences with high efficiency and accuracy.