公开(公告)号：US20220186263A1

公开(公告)日：2022-06-16

申请号：US17601303

申请日：2020-04-03

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Yoshihiro UNO ,  Yuko KOTANI ,  Yoshiki MIYASAKA ,  Yuichiro OKA ,  Makoto SATO

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/10

Abstract: The present inventors have found that the use of a site-specific nuclease system which includes a combination of a molecule that simultaneously targets and cleaves one homology arm sequence of the donor DNA and the genomic sequence corresponding to the homology arm sequence, and a molecule that targets and cleaves the genomic region in the vicinity of the cleavage site by that molecule causes repair between genomic DNA and donor DNA through both non-homologous end joining and homologous recombination, making it possible to produce cells and organisms with knocked-in long donor sequences with high efficiency and accuracy.

公开(公告)号：US20240124898A1

公开(公告)日：2024-04-18

申请号：US18467297

申请日：2023-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Junji TAKEDA ,  Hiroyuki MORISAKA ,  Kazuto YOSHIMI

IPC: C12N15/90 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85

CPC classification number: C12N15/907 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85 ,  C12N15/8509 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

公开(公告)号：US20230099483A1

公开(公告)日：2023-03-30

申请号：US17794704

申请日：2021-01-25

Applicant: C4U CORPORATION ,  OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Kazuto YOSHIMI ,  Satomi SHIBUMURA

IPC: C12Q1/70

Abstract: It has been found that by mixing a single-stranded probe DNA whose cleavage can be detected in a reaction system containing a CRISPR-Cas3 system and a sample from which to detect a target DNA, it is possible to detect the target DNA in the sample by using, as indication, a signal generated by the cleavage of the single-stranded probe DNA.

公开(公告)号：US20200029538A1

公开(公告)日：2020-01-30

申请号：US16463883

申请日：2017-11-24

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Yoshiki MIYASAKA

IPC: A01K67/027 ,  C12N15/90 ,  C12N15/10

Abstract: A method for producing a genome edited cell or a non-human organism, comprising the step of introducing (a) an artificial nuclease system which cleaves both ends of a genome editing target region, and (b) a nucleic acid sequence formed by arranging a 5′-side homology arm sequence, a donor DNA sequence, and a 3′-side homology arm sequence in this order from a 5′-side, the 5′-side homology arm sequence being a homologous sequence of one nucleic acid sequence outside the genome editing target region, and the 3′-side homology arm sequence being a homologous sequence of the other nucleic acid sequence outside the genome editing target region, into a cell or a non-human organism.

公开(公告)号：US20240117381A1

公开(公告)日：2024-04-11

申请号：US18467356

申请日：2023-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji MASHIMO ,  Junji TAKEDA ,  Hiroyuki MORISAKA ,  Kazuto YOSHIMI

IPC: C12N15/90 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85

CPC classification number: C12N15/907 ,  A61K48/00 ,  C12N5/14 ,  C12N5/16 ,  C12N9/22 ,  C12N15/85 ,  C12N15/8509 ,  C12N2310/20 ,  C12N2800/22 ,  C12N2800/80

Abstract: A CRISPR-Cas3 system was successfully established in a eukaryotic cell.

公开(公告)号：US20220017888A1

公开(公告)日：2022-01-20

申请号：US17309607

申请日：2019-12-11

Applicant: Kyoto University ,  Osaka University

Inventor： Akitsu HOTTA ,  Yuya OKUZAKI ,  Huaigeng XU ,  Peter David GEE ,  Yuto KITA ,  Tomoji MASHIMO ,  Kazuto YOSHIMI

IPC: C12N15/10 ,  C12N9/22

Abstract: A production method of genomic DNA in which a deletion of more than 100 bases of nucleotides is introduced into a target region of the genomic DNA, the method includes a contact step of bringing a type I CRISPR associated complex for anti-viral defense (type I Cascade complex), CRISPR RNA (crRNA), and Cas3 protein into contact with the genomic DNA.