-
公开(公告)号：US09797009B2
公开(公告)日：2017-10-24
申请号：US14415533
申请日：2013-07-18
发明人： Andrew Heron , James Clarke , Ruth Moysey , Elizabeth Jayne Wallace , Mark Bruce , Lakmal Jayasinghe , Domenico Caprotti , Szabolcs Soeroes , Luke McNeill , Mihaela Misca
CPC分类号： C12Q1/6869 , C07K2319/00 , C12N9/14 , C12Y306/04 , C12Q2521/513 , C12Q2522/101 , C12Q2565/631
摘要： The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
-
公开(公告)号：US20150218629A1
公开(公告)日：2015-08-06
申请号：US14415533
申请日：2013-07-18
发明人： Andrew Heron , James Clarke , Ruth Moysey , Elizabeth Jayne Wallace , Mark Bruce , Lakmal Jayasinghe , Domenico Caprotti , Szabolcs Soeroes , Luke McNeill , Mihaela Misca
CPC分类号： C12Q1/6869 , C07K2319/00 , C12N9/14 , C12Y306/04 , C12Q2521/513 , C12Q2522/101 , C12Q2565/631
摘要： The invention relates to methods using constructs comprising a helicase and an additional polynucleotide binding moiety. The helicase is attached to the polynucleotide binding moiety and the construct has the ability to control the movement of a polynucleotide. The constructs can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要翻译： 本发明涉及使用包含解旋酶和另外的多核苷酸结合部分的构建体的方法。 解旋酶连接到多核苷酸结合部分，并且构建体具有控制多核苷酸运动的能力。 该构建体可用于控制多核苷酸的移动，并且特别可用于测序多核苷酸。
-
3.发明申请公开(公告)号：US20150065354A1
公开(公告)日：2015-03-05
申请号：US14369024
申请日：2012-12-28
发明人： Ruth Moysey , Andrew John Heron , Szabolcs Soeroes
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/513 , C12Q2527/137 , C12Q2565/607 , C12Q2565/631
摘要： The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and an XPD helicase. The helicase controls the movement of the target polynucleotide through the pore.
摘要翻译： 本发明涉及表征靶多核苷酸的新方法。 该方法使用孔和XPD解旋酶。 解旋酶控制靶多核苷酸通过孔的运动。
-
公开(公告)号：US20140335512A1
公开(公告)日：2014-11-13
申请号：US14369072
申请日：2012-12-28
发明人： Ruth Moysey , Andrew John Heron , Szabolcs Soeroes
CPC分类号： C12Q1/68 , C12N9/14 , C12Q1/6869 , C12Y306/04012 , C12Q2521/513 , C12Q2563/116 , C12Q2565/631
摘要： The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a RecD helicase. The helicase controls the movement of the target polynucleotide through the pore.
摘要翻译： 本发明涉及表征靶多核苷酸的新方法。 该方法使用孔和RecD解旋酶。 解旋酶控制靶多核苷酸通过孔的运动。
-
公开(公告)号：US20210123032A1
公开(公告)日：2021-04-29
申请号：US17064329
申请日：2020-10-06
发明人： Andrew Heron , Anthony Clarke , Ruth Moysey , Elizabeth Jayne Wallace , Mark Bruce , Lakmal Jayasinghe , Domenico Caprotti , Szabolcs Soeroes , Luke McNeill , David Alves , Rebecca Bowen , John Milton
IPC分类号： C12N9/14 , C12N9/90 , C12Q1/6827
摘要： The invention relates to modified helicases with reduced unbinding from polynucleotides. The helicases can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
-
公开(公告)号：US09758823B2
公开(公告)日：2017-09-12
申请号：US14351038
申请日：2012-10-18
发明人： Ruth Moysey , Andrew John Heron
CPC分类号： C12Q1/6869 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
-
公开(公告)号：US20140255921A1
公开(公告)日：2014-09-11
申请号：US14351038
申请日：2012-10-18
发明人： Ruth Moysey , Andrew John Heron
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
摘要翻译： 本发明涉及表征靶多核苷酸的新方法。 该方法使用孔和Hel308解旋酶或分子运动，其能够在内部核苷酸上结合靶多核苷酸。 解旋酶或分子运动通过孔控制靶多核苷酸的运动。
-
公开(公告)号：US20220145383A1
公开(公告)日：2022-05-12
申请号：US17481374
申请日：2021-09-22
发明人： James White , Ruth Moysey , Mihaela Misca
IPC分类号： C12Q1/6869
摘要： The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
-
公开(公告)号：US11155860B2
公开(公告)日：2021-10-26
申请号：US14415459
申请日：2013-07-18
发明人： James White , Ruth Moysey , Mihaela Misca
IPC分类号： C12Q1/6869
摘要： The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
-
公开(公告)号：US09617591B2
公开(公告)日：2017-04-11
申请号：US14369024
申请日：2012-12-28
发明人： Ruth Moysey , Andrew John Heron , Szabolcs Soeroes
CPC分类号： C12Q1/6869 , C12Q2521/513 , C12Q2527/137 , C12Q2565/607 , C12Q2565/631
摘要： The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and an XPD helicase. The helicase controls the movement of the target polynucleotide through the pore.
-
-
-
-
-
-
-
-
-
搜索结果
16 条记录 (耗时 0.026 秒)
