公开(公告)号：US10197552B2

公开(公告)日：2019-02-05

申请号：US14981076

申请日：2015-12-28

申请人： Oakland University

发明人： Xiangqun Zeng ,  Gerard Madlambayan ,  Liang Tan ,  Bahareh Pezeshkian ,  Abdul Rehman

IPC分类号： G01N29/02 ,  G01N33/483 ,  G01N29/42 ,  G01N29/036 ,  G01N29/44

摘要： In a method for monitoring cell-to-cell interactions, a quartz crystal microbalance surface is exposed to a medium including a first cell. The first cell is exposed to a sample including a suspect cell. The first cell is activated prior to or simultaneously with the first cell exposure. Frequency and motional resistance changes versus time are measured after each of: surface exposure to the medium, first cell activation prior to the exposure to the sample, and first cell exposure to the sample; or after each of: surface exposure to the medium and simultaneous first cell activation and sample exposure. From the frequency and motional resistance changes versus time, any of i) a level of adhesion of the suspect cell to the activated first cell, ii) a type of the suspect cell, iii) a behavior or activity of the suspect cell is determined, or iv) activation of the first cell is determined.

公开(公告)号：US20160202235A1

公开(公告)日：2016-07-14

申请号：US14981076

申请日：2015-12-28

申请人： Oakland University

发明人： Xiangqun Zeng ,  Gerard Madlambayan ,  Liang Tan ,  Bahareh Pezeshkian ,  Abdul Rehman

IPC分类号： G01N33/483 ,  G01N29/42

CPC分类号： G01N33/4833 ,  G01N29/022 ,  G01N29/036 ,  G01N29/42 ,  G01N29/4472 ,  G01N2291/0228 ,  G01N2291/02466 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0426

摘要： In a method for monitoring cell-to-cell interactions, a quartz crystal microbalance surface is exposed to a medium including a first cell. The first cell is exposed to a sample including a suspect cell. The first cell is activated prior to or simultaneously with the first cellexposure. Frequency and motional resistance changes versus time are measured after each of: surface exposure to the medium, first cell activation prior to the exposure to the sample, and first cell exposure to the sample; or after each of: surface exposure to the medium and simultaneous first cell activation and sample exposure. From the frequency and motional resistance changes versus time, any of i) a level of adhesion of the suspect cell to the activated first cell, ii) a type of the suspect cell, iii) a behavior or activity of the suspect cell is determined, or iv) activation of the first cell is determined.

摘要翻译： 在用于监测细胞间细胞相互作用的方法中，将石英晶体微量平衡表面暴露于包括第一细胞的培养基。 将第一个细胞暴露于包含可疑细胞的样品。 第一个细胞在第一次曝光之前或同时被激活。 在暴露于样品之前的表面暴露于介质，第一细胞活化和第一细胞暴露于样品之后，测量频率和运动电阻变化对时间的影响。 或者之后：表面暴露于培养基和同时进行第一细胞活化和样品暴露。 从频率和运动阻力变化对时间，i）可疑细胞与活化的第一细胞的粘附水平，ii）可疑细胞的类型，iii）确定可疑细胞的行为或活性， 或iv）确定第一个细胞的活化。