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公开(公告)号:US20090246876A1
公开(公告)日:2009-10-01
申请号:US12410495
申请日:2009-03-25
申请人: PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , CAROL M. MCCUTCHEN , MARY ANN FRANDEN
发明人: PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , CAROL M. MCCUTCHEN , MARY ANN FRANDEN
CPC分类号: C12N15/74
摘要: Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
摘要翻译: 鉴定为运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的启动子的突变体,其直接改善可操作地连接的异源核酸的表达水平。 这些是用于在发酵单胞菌,酵母属和其他相关细菌中表达嵌合基因的高表达启动子。
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公开(公告)号:US20090246846A1
公开(公告)日:2009-10-01
申请号:US12410501
申请日:2009-03-25
申请人: PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , CAROL M. MCCUTCHEN , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , MARY ANN FRANDEN
发明人: PAUL V. VIITANEN , LUAN TAO , YUYING ZHANG , PERRY G. CAIMI , CAROL M. MCCUTCHEN , LAURA MCCOLE , MIN ZHANG , YAT-CHEN CHOU , MARY ANN FRANDEN
CPC分类号: C12N9/92 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12P7/065 , Y02E50/17
摘要: Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.
摘要翻译: 通过引入含有运动发酵单胞菌甘油醛-3-磷酸脱氢酶基因的突变启动子的嵌合木糖异构酶基因工程化发酵单胞菌菌株。 启动子指导木糖异构酶的增加表达,并且当该菌株另外被设计用于表达木酮糖激酶,转醛醇酶和转酮酶时,获得木糖的改善的利用。
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