公开(公告)号：US12121588B2

公开(公告)日：2024-10-22

申请号：US16480792

申请日：2018-02-01

Applicant: PFIZER INC.

Inventor： Kaushik Dutta ,  Jose Manuel Gomes ,  Frank W. Kotch ,  Vimalkumar B. Patel ,  Amarnauth Shastrie Prashad ,  Renee L. Procopio-Melino ,  Xiaotian Zhong

IPC: A61K47/42 ,  A61K47/50 ,  A61K47/64 ,  A61K47/68 ,  C07B31/00 ,  C07B33/00 ,  C07K1/00 ,  C07K16/30 ,  C07K1/107 ,  C07K1/20

CPC classification number: A61K47/6843 ,  A61K47/6855 ,  A61K47/6889 ,  C07K1/00 ,  C07K16/3015 ,  C07K1/1077 ,  C07K1/20 ,  C07K2317/14

Abstract: Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs).; conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.