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    Systems and methods for cellular reprogramming of a plant cell
    2.
    发明授权
    Systems and methods for cellular reprogramming of a plant cell 有权

    公开(公告)号：US11447786B2

    公开(公告)日：2022-09-20

    申请号：US16755432

    申请日：2018-10-12

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Tim Fox ,  William James Gordon-Kamm ,  Rachel Carol Huegel ,  Keith S Lowe ,  Jon Aaron Tucker Reinders ,  Huaxun Ye

    IPC分类号： C12N15/82 ,  A01H1/08 ,  C12N9/22

    摘要： Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

    Use of morphogenic factors for the improvement of gene editing
    5.
    发明授权
    Use of morphogenic factors for the improvement of gene editing 有权

    公开(公告)号：US11332752B2

    公开(公告)日：2022-05-17

    申请号：US16979917

    申请日：2019-03-11

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Stephen Lawrence Gasior ,  William James Gordon-Kamm ,  Keith S Lowe ,  David J Peterson ,  Sergei Svitashev ,  Joshua K Young

    IPC分类号： C12N15/82

    摘要： Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.