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14 条记录 (耗时 0.028 秒)

    Use of morphogenic factors for the improvement of gene editing
    5.
    发明授权
    Use of morphogenic factors for the improvement of gene editing 有权

    公开(公告)号：US11332752B2

    公开(公告)日：2022-05-17

    申请号：US16979917

    申请日：2019-03-11

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Stephen Lawrence Gasior ,  William James Gordon-Kamm ,  Keith S Lowe ,  David J Peterson ,  Sergei Svitashev ,  Joshua K Young

    IPC分类号： C12N15/82

    摘要： Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.

    Methods and compositions for accelerated trait introgression
    7.
    发明授权
    Methods and compositions for accelerated trait introgression 有权

    公开(公告)号：US11492632B2

    公开(公告)日：2022-11-08

    申请号：US15554234

    申请日：2016-03-16

    申请人： PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Michael T Elsing ,  William James Gordon-Kamm ,  Leandro Daniel Perugini ,  Huaxun Ye

    IPC分类号： C12N15/82 ,  A01H1/04

    摘要： Compositions and methods are provided for the use of pollen-inhibitor genes and/or color marker genes in accelerated trait introgression. Compositions and methods are also provided for introducing a pollen-inhibitor gene and/or a color marker gene in close proximity to a trait locus of interest. Breeding methods and methods for selecting plants comprising a trait locus of interest in close proximity to at least one pollen-inhibitor gene and/or color marker gene are also disclosed. The methods and compositions employ at least one pollen-inhibitor gene and/or color marker gene to provide an effective system for accelerated trait introgression in the genome of a plant.

    WUSCHEL (WUS) GENE HOMOLOGS
    9.
    发明申请
    WUSCHEL (WUS) GENE HOMOLOGS 审中-公开
    WUSCHEL（WUS）基因同源物

    公开(公告)号：US20130139279A1

    公开(公告)日：2013-05-30

    申请号：US13744024

    申请日：2013-01-17

    申请人： E.I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

    发明人： Rebecca E. Cahoon ,  William James Gordon-Kamm ,  Keith S. Lowe ,  Christopher Jay Scelonge ,  Yumin Tao

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8241 ,  C07K14/415 ,  C12N15/62 ,  C12N15/8201 ,  C12N15/8207 ,  C12N15/8261 ,  Y02A40/146

    摘要： This invention relates to isolated polynucleotides encoding WUS polypeptides. The invention further provides isolated WUS polypeptides. The invention also provides methods of using the polynucleotides to modulate the level of WUS, improve transformation efficiency, to stimulate plant cell growth, including stem cells, to stimulate organogenesis, to stimulate somatic embryogenesis, to induce apomixis, and to provide a positive selection for cells comprising the polynucleotide. The invention also relates to cells, plants and seeds comprising the polynucleotides of the invention or produced by the methods of the invention.

    摘要翻译： 本发明涉及编码WUS多肽的分离的多核苷酸。 本发明还提供了分离的WUS多肽。 本发明还提供了使用多核苷酸来调节WUS水平，提高转化效率，刺激植物细胞生长（包括干细胞）刺激器官发生，刺激体细胞胚发生，诱导无融合生殖以及提供阳性选择的方法 包含多核苷酸的细胞。 本发明还涉及包含本发明的多核苷酸或通过本发明的方法产生的细胞，植物和种子。