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1.发明公开公开(公告)号：US20240360470A1
公开(公告)日：2024-10-31
申请号：US18765988
申请日：2024-07-08
发明人： UTE BAUMANN , ANDREW MARK CIGAN , MARGARET ANNE PALLOTTA , MANJIT SINGH , RADOSLAW SUCHECKI , NATHAN SPENCER WATSON-HAIGH , RYAN WHITFORD
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8213
摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant.
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公开(公告)号：US20200087678A1
公开(公告)日：2020-03-19
申请号：US16697681
申请日：2019-11-27
发明人： MARC C ALBERTSEN , UTE BAUMANN , ANDREW MARK CIGAN , MANJIT SINGH , ELISE TUCKER , RYAN WHITFORD
IPC分类号： C12N15/82
摘要： Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.
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3.发明公开公开(公告)号：US20230183738A1
公开(公告)日：2023-06-15
申请号：US18064423
申请日：2022-12-12
发明人： ANDREW MARK CIGAN , MANJIT SINGH
IPC分类号： C12N15/82 , C07K14/415 , A01H1/02
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8231 , A01H1/023
摘要： Compositions and methods are provided for screening wheat seed for sorting and selection. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which encode and express a screenable color marker in seeds. Expression cassettes comprise a plant-derived polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the color, opacity, fluorescence, or other property of the seed. The plant-derived marker can be used in a male-sterile production system of hybrid wheat seed. Methods for maintaining a line of male-sterile plants and for restoring male fertility in a male-sterile plant, comprising a screenable color marker are provided.
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公开(公告)号：US20220056439A1
公开(公告)日：2022-02-24
申请号：US17454510
申请日：2021-11-11
发明人： ANDREW MARK CIGAN , TIM FOX , MANJIT SINGH
IPC分类号： C12N15/11 , C12N9/22 , C12N15/82 , C07K14/415
摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.
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公开(公告)号：US20220056472A1
公开(公告)日：2022-02-24
申请号：US17275111
申请日：2019-09-13
发明人： MARC C ALBERTSEN , TIM FOX , MANJIT SINGH , MARK E WILLIAMS
IPC分类号： C12N15/82
摘要： Provided herein are methods and compositions for restoring fertility and maintaining sterility in plants. In particular, one method disclosed includes introducing into a male-sterile plant, wherein the plant comprises one or more homozygous mutations in a male-fertility gene, a plant restoration donor chromosomal component comprising a plant-derived polynucleotide that confers a plant phenotypic marker linked to a male-fertility restoration locus that functionally complements the male-sterility phenotype from the one or more homozygous mutations in the male-sterile plant. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker and the male-fertility restoration locus are linked to each other and located on the same chromosomal arm on the plant restoration donor chromosomal component. In some examples, the plant-derived polynucleotide that confers a plant phenotypic marker, the male-fertility restoration locus, or both are modified.
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公开(公告)号：US20170369902A1
公开(公告)日：2017-12-28
申请号：US15536336
申请日：2015-12-15
发明人： ANDREW MARK CIGAN , MANJIT SINGH
IPC分类号： C12N15/82 , C07K14/415 , A01H5/10 , A01H1/02
CPC分类号： C12N15/8289 , A01H1/02 , A01H5/10 , C07K14/415 , C12N9/0077 , C12N15/01 , C12N15/8213
摘要： Manipulation of male fertility in a polyploid species requires attention to the interaction of male-fertility alleles of multiple genomes. In hexaploid wheat, single-genome heterozygotes for Ms26 provide differential levels of male fertility across genomes. Hexaploid wheat homozygous for mutations in the Ms26 gene on the A, B, and D genomes is male-sterile. Male fertility may be restored by sufficient levels of expression of Ms26 using native Ms26 or a transgene, which may be native to wheat or to another species, or a combination of native and transgenic alleles. CRISPR/Cas9 technology may be used to generate mutations in Ms26 in wheat or rice.
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公开(公告)号：US20210254096A1
公开(公告)日：2021-08-19
申请号：US17244603
申请日：2021-04-29
发明人： MARC C ALBERTSEN , UTE BAUMANN , ANDREW MARK CIGAN , MANJIT SINGH , ELISE TUCKER , RYAN WHITFORD
IPC分类号： C12N15/82
摘要： Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.
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8.发明申请公开(公告)号：US20190177722A1
公开(公告)日：2019-06-13
申请号：US16214897
申请日：2018-12-10
发明人： ANDREW MARK CIGAN , TIM FOX , MANJIT SINGH
摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.
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公开(公告)号：US20170298383A1
公开(公告)日：2017-10-19
申请号：US15514195
申请日：2015-09-21
发明人： MARC C. ALBERTSEN , UTE BAUMANN , ANDREW MARK CIGAN , MANJIT SINGH , ELISE TUCKER , RYAN WHITFORD
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8289 , A01H1/02 , A01H5/10 , C07K14/415 , C12N15/8213 , C12N15/8231 , C12N15/8287 , C12Q1/6895 , C12Q2600/13
摘要： Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.
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10.发明公开公开(公告)号：US20240254478A1
公开(公告)日：2024-08-01
申请号：US18437343
申请日：2024-02-09
发明人： ANDREW MARK CIGAN , TIM FOX , MANJIT SINGH
IPC分类号： C12N15/11 , C07K14/415 , C12N9/22 , C12N15/82
CPC分类号： C12N15/11 , C07K14/415 , C12N9/22 , C12N15/8213 , C12N15/8289 , C12N2310/20 , C12N2800/80
摘要： Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of MS9, MS22, MS26, or MS45 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms9, Ms22, Ms26, or Ms45 nucleotide sequence to a male-sterile Ms9, Ms22, Ms26, or Ms45 plant produced using the methods and compositions described herein.
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