公开(公告)号：US20240327805A1

公开(公告)日：2024-10-03

申请号：US18744124

申请日：2024-06-14

申请人： Kevin Redding ,  Andrey Kanygin

发明人： Kevin Redding ,  Andrey Kanygin

IPC分类号： C12N9/02 ,  C07K14/405 ,  C12N1/12 ,  C12N15/62 ,  C12P3/00

CPC分类号： C12N9/0077 ,  C12N1/12 ,  C12N15/62 ,  C12P3/00 ,  C07K14/405 ,  C07K2319/00

摘要： Provided herein, in some embodiments, are engineered cells and use of the same for increased hydrogen production. In particular, provided herein are genetically engineered cells comprising a polynucleotide encoding a fusion protein comprising a photosystem I (PSI) protein and an algal hydrogenase, as well as methods for producing such genetically engineered cells. Also provided herein are methods for increasing hydrogen (H2) production in cells.

公开(公告)号：US11913049B2

公开(公告)日：2024-02-27

申请号：US16878032

申请日：2020-05-19

申请人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

发明人： Ramon Gonzalez ,  James Clomburg ,  Alexander Chou

IPC分类号： C12N9/00 ,  C12P19/32 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/46 ,  C12N9/04 ,  C12N9/02 ,  C12P7/625 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/42 ,  C12P9/00 ,  C12P13/00 ,  C12P13/04

CPC分类号： C12P19/32 ,  C12N9/00 ,  C12N9/001 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0077 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/93 ,  C12P5/02 ,  C12P5/026 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/625 ,  C12P9/00 ,  C12P13/001 ,  C12P13/04 ,  C12Y101/01 ,  C12Y101/03039 ,  C12Y102/01 ,  C12Y102/04001 ,  C12Y103/05001 ,  C12Y103/99 ,  C12Y108/01004 ,  C12Y114/15003 ,  C12Y203/01012 ,  C12Y203/01054 ,  C12Y207/01031 ,  C12Y208/03 ,  C12Y401/00 ,  C12Y401/01 ,  C12Y401/01047 ,  C12Y401/03024 ,  C12Y402/01 ,  C12Y402/01002 ,  C12Y402/01011 ,  C12Y501/99001 ,  C12Y501/99002 ,  C12Y504/02 ,  C12Y504/99 ,  C12Y504/99001 ,  C12Y504/99002 ,  C12Y602/01 ,  C12Y602/01005 ,  C12Y604/01003 ,  Y02E50/30

摘要： An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane.

公开(公告)号：US20240052434A1

公开(公告)日：2024-02-15

申请号：US17641791

申请日：2020-09-09

申请人： Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.

发明人： Joseph Hirschberg ,  Daniel Zamir ,  Uri Karniel ,  Amit Koch

IPC分类号： C12Q1/6895 ,  C12N9/90 ,  C12N9/02 ,  C12N15/82

CPC分类号： C12Q1/6895 ,  C12N9/90 ,  C12Y505/01019 ,  C12N9/0073 ,  C12Y114/1309 ,  C12N9/0077 ,  C12N15/8245 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The present invention relates to tomato plants having fruit with elevated nutritional and health benefits, particularly fruit with elevated content of the carotenoid zeaxanthin, a carotenoid offering a range of health benefits, particularly thought to inhibit the progression of age-related macular degradation (AMD), the most prevalent cause of blindness in developed countries.

公开(公告)号：US20230250456A1

公开(公告)日：2023-08-10

申请号：US18014674

申请日：2021-07-07

申请人： VIO CHEMICALS AG

发明人： Sven PANKE ,  Martin HELD ,  Tsvetan KARDASHLIEV

IPC分类号： C12P17/12 ,  C07D213/30 ,  C12N9/02 ,  C12N15/70 ,  C12N1/20 ,  C12N9/04

CPC分类号： C12P17/12 ,  C07D213/30 ,  C12N9/0077 ,  C12N9/0093 ,  C12N15/70 ,  C12N1/20 ,  C12N9/0006 ,  C12Y101/01021 ,  C12Y207/01017 ,  C12Y101/01001 ,  C12Y117/01

摘要： The present invention relates to the provision of an enzymatic method for the preparation of 2,6-bis(hydroxymethyl) pyridine (Formula I) using as substrate 2,6-Dimethlypyridine (2,6-lutidene) and the multicomponent xylene monooxygenase comprising XylM and XylA from Pseudomonas putida (Arthrobacter siderocapsulatus). The enzymatic method of the present invention is advantageous over conventional synthetic preparations, providing access to the title compound with a one-step enzymatic procedure.

公开(公告)号：US20230203518A1

公开(公告)日：2023-06-29

申请号：US17719820

申请日：2022-04-13

申请人： U.S. Smokeless Tobacco Company LLC

发明人： Dongmei XU ,  Mark T. Nielsen ,  Yanxin Shen

IPC分类号： C12N15/82 ,  A01H6/82 ,  A01H5/12 ,  A24B15/20 ,  C12N9/02

CPC分类号： C12N15/8243 ,  A01H5/12 ,  A01H6/823 ,  A24B15/20 ,  C12N9/0073 ,  C12N9/0077 ,  C12N15/8218

摘要： The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

公开(公告)号：US20230151393A1

公开(公告)日：2023-05-18

申请号：US16766225

申请日：2019-01-16

申请人： FIRMENICH SA

发明人： Michel Schalk ,  Letizia Rocci ,  Fabienne Deguerry

IPC分类号： C12P7/02 ,  C12N5/10 ,  C12N15/63 ,  C12N15/52 ,  C12N9/02 ,  C12P5/00

CPC分类号： C12P7/02 ,  C12N5/10 ,  C12N15/63 ,  C12N15/52 ,  C12N9/0077 ,  C12Y114/15006 ,  C12P5/007

摘要： Described herein are the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. Also described herein are methods of oxidizing terpene molecules including contacting the cytochrome P450 with the terpene molecule intended to be oxidized. In particular, the method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. Also described herein is an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the disclosure.

公开(公告)号：US20190045812A1

公开(公告)日：2019-02-14

申请号：US16050012

申请日：2018-07-31

申请人： Industry-University Cooperation Foundation Hanyang University

发明人： Eon Seon JIN ,  Sang Su BAE ,  Kwang Ryul BAEK ,  Duk Hyoung KIM ,  Joo Yeon JEONG

IPC分类号： A23K20/179 ,  A61K9/00 ,  C12R1/89

CPC分类号： A23K20/179 ,  A23K10/16 ,  A23K20/174 ,  A23L29/065 ,  A23L33/135 ,  A61K9/0053 ,  A61K9/2018 ,  C12N1/12 ,  C12N9/0073 ,  C12N9/0077 ,  C12P23/00 ,  C12R1/89 ,  C12Y114/1309 ,  C12Y114/15

摘要： The present invention relates to a new alga having an improved ability to produce a pigment, and when a mutant of the present invention is used, a carotenoid-based pigment, specifically, a xanthophyll can be produced by consuming less energy, so that it is possible to efficiently produce the pigment at the industrial level. Further, the pigment can be applied as a raw material for a food, a health functional food and a medicine, which include the pigment. In particular, since a DNA fragment is not likely to be inserted into a target base sequence or a base sequence other than the target, it is expected that the procedure of constructing the mutant is not regulated as a GMO, so that it is expected that the procedure of constructing the mutant can create a big economic effect in terms of an industry which produces lutein and zeaxanthin by using microalgae.

公开(公告)号：US20180273983A1

公开(公告)日：2018-09-27

申请号：US15942001

申请日：2018-03-30

申请人： The California Institute of Technology

发明人： Frances Arnold ,  Peter Meinhold ,  Matthew W. Peters ,  Rudi Fasan ,  Mike M.Y. Chen

IPC分类号： C12P7/04 ,  C12N9/02 ,  C12P7/06

CPC分类号： C12P7/04 ,  C07K14/32 ,  C12N9/0042 ,  C12N9/0071 ,  C12N9/0077 ,  C12P7/06 ,  C12Y106/02004 ,  C12Y114/14001 ,  C12Y114/15 ,  C12Y114/15003 ,  Y02E50/17 ,  Y02P20/52

摘要： This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

公开(公告)号：US20180265897A1

公开(公告)日：2018-09-20

申请号：US15540176

申请日：2015-12-30

申请人： EVOLVA SA ,  University of Copenhagen

发明人： Birger Lindberg Møller ,  Bjorn Hamberger ,  Roberta Callari ,  Dan Luo ,  Morten Thrane Nielsen

IPC分类号： C12P5/00 ,  C12N15/82 ,  C12N9/04 ,  C12N9/10

CPC分类号： C12P5/002 ,  C12N9/0006 ,  C12N9/0077 ,  C12N9/1022 ,  C12N9/1085 ,  C12N9/88 ,  C12N15/8243 ,  C12Y101/01001 ,  C12Y202/01007 ,  C12Y205/01029

摘要： The invention relates to recombinant microorganisms and methods for producing macrocyclic diterpene or oxidized macrocyclic diterpene.

公开(公告)号：US20180201943A1

公开(公告)日：2018-07-19

申请号：US15855237

申请日：2017-12-27

申请人： Board of Regents, The University of Texas System

发明人： John Blazeck ,  Andrew Hill ,  Leqian Liu ,  Hal Alper

IPC分类号： C12N15/81 ,  C12R1/645 ,  C07K14/39 ,  C12P7/64 ,  C12N15/52 ,  C12N15/01 ,  C12N9/00 ,  C12N9/88 ,  C12N9/78 ,  C12N9/10 ,  C12N9/02 ,  C12N9/04 ,  C12N1/16

CPC分类号： C12N15/815 ,  C07K14/39 ,  C12N1/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0042 ,  C12N9/0077 ,  C12N9/1007 ,  C12N9/78 ,  C12N9/88 ,  C12N9/93 ,  C12N15/01 ,  C12N15/52 ,  C12P7/64 ,  C12P7/6409 ,  C12P7/6463 ,  C12R1/645

摘要： Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals.