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公开(公告)号：US11959085B2
公开(公告)日：2024-04-16
申请号：US16797472
申请日：2020-02-21
发明人： Scott Diehn , Michelle Van Allen , Albert L Lu
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8222 , C12N2730/00022 , C12N2730/00043
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Lamium Leaf Distortion Associated Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.
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公开(公告)号：US11702668B2
公开(公告)日：2023-07-18
申请号：US17056219
申请日：2019-05-20
发明人： Andrew Carl Crow , Scott Diehn , Lynne Eileen Sims
IPC分类号： C12N15/82
CPC分类号： C12N15/8223 , C12N15/823 , C12N15/8286
摘要： The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
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公开(公告)号：US11325949B2
公开(公告)日：2022-05-10
申请号：US16841168
申请日：2020-04-06
发明人： Scott Diehn , James J English , Lu Liu , Azalea S Ong , Jarred Kenneth Oral , Barbara Rosen , Ute Schellenberger , Ingrid Udranszky , Jun-Zhi Wei , Weiping Xie , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US20140283206A1
公开(公告)日：2014-09-18
申请号：US13795118
申请日：2013-03-12
IPC分类号： C12N15/82
CPC分类号： A01H5/10 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor pLTP. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码高粱双色pLTP的基因的启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。
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公开(公告)号：US12065653B2
公开(公告)日：2024-08-20
申请号：US16311893
申请日：2017-06-08
发明人： Andrew Crow , Scott Diehn , Lynne Sims
CPC分类号： C12N15/8227 , C12N5/04 , C12N15/62 , C12N15/8216
摘要： The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
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公开(公告)号：US09920316B2
公开(公告)日：2018-03-20
申请号：US13831230
申请日：2013-03-14
发明人： Xu Hu , James Presnail , Nina Richtman , Scott Diehn , Michelle Van Allen , Lisa Procyk
IPC分类号： C12N15/82 , C12N15/113 , A01N37/46 , A01N63/02
CPC分类号： C12N15/113 , A01N37/46 , A01N63/02 , C12N15/8218 , C12N15/8286 , C12N2310/14 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: disclosed herein, (but not including the forward and reverse primers.) or active variants and fragments thereof, or complements thereof, wherein a decrease in expression of one or more of the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiments, the pest is D. virgifera virgifera, D. barberi, D. virgifera zeae, D. speciosa, D. speciosa, or D. undecimpunctata howardi. Plants, plant parts, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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公开(公告)号：US20140283205A1
公开(公告)日：2014-09-18
申请号：US13794917
申请日：2013-03-12
IPC分类号： C12N15/82
CPC分类号： C12N15/8227 , C12N15/8241
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor TIP2-3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码高粱双色TIP2-3的基因的启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。
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公开(公告)号：US10667524B2
公开(公告)日：2020-06-02
申请号：US15022109
申请日：2014-09-11
发明人： Scott Diehn , James English , Lu Liu , Azalea Ong , Jarred Oral , Barbara Rosen , Ute Schellenberger , Ingrid Udranszky , Jun-Zhi Wei , Weiping Xie , Genhai Zhu
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
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公开(公告)号：US20190136331A1
公开(公告)日：2019-05-09
申请号：US16246608
申请日：2019-01-14
发明人： Scott Diehn , Albert L Lu , Timothy M Nowatzki , Douglas S Nubel , M Alejandra Pascual , James C Register, III , Christopher J Scelonge , Joshua K Young , Cathy Xiaoyan Zhong , Erin L Crowgey
IPC分类号： C12Q1/6895 , A01H5/10 , C12N15/82
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
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公开(公告)号：US09273322B2
公开(公告)日：2016-03-01
申请号：US13795118
申请日：2013-03-12
CPC分类号： A01H5/10 , C12N15/8227
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor pLTP. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.
摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物包括用于编码高粱双色pLTP的基因的启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括用与本发明的启动子之一可操作地连接的核苷酸序列转化植物或植物细胞。
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