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1.发明申请公开(公告)号：US20210180077A1
公开(公告)日：2021-06-17
申请号：US17181136
申请日：2021-02-22
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE , KEVIN E MCBRIDE , CHRISTOPHER J SCELONGE , NING WANG
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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2.发明公开公开(公告)号：US20230265448A1
公开(公告)日：2023-08-24
申请号：US18047714
申请日：2022-10-19
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE , KEVIN E MCBRIDE , CHRISTOPHER J SCELONGE , NING WANG
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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公开(公告)号：US20230124856A1
公开(公告)日：2023-04-20
申请号：US17907040
申请日：2021-03-23
摘要： Compositions and methods for genome editing in sunflower are provided. Exemplary compositions include constructs for Cas endonuclease mediated genome editing of the FAD2-1 locus as well as sunflower plants, seeds and cells thereof which are modified using the disclosed compositions.
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公开(公告)号：US20190194678A1
公开(公告)日：2019-06-27
申请号：US15956794
申请日：2018-04-19
发明人： WILLIAM J GORDON-KAMM , TIMOTHY G HELENTJARIS , KEITH S LOWE , BO SHEN , MITCHELL C TARCZYNSKI , PEIZHONG ZHENG
IPC分类号： C12N15/82 , C07K14/415 , A01H1/08
CPC分类号： C12N15/8243 , A01H1/08 , C07K14/415 , C12N15/8201 , C12N15/8205 , C12N15/8209 , C12N15/8223 , C12N15/8231 , C12N15/8233 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
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公开(公告)号：US20220124998A1
公开(公告)日：2022-04-28
申请号：US17571428
申请日：2022-01-07
发明人： AJITH ANAND , MAREN L ARLING , ALEXANDRE DA SILVA CONCEICAO , WILLIAM JAMES GORDON-KAMM , CRAIG HASTINGS , GEORGE J HOERSTER , TODD J JONES , THEODORE M KLEIN , CARLOS M LA ROTA , KEITH S LOWE , SHIV BAHADUR TIWARI , NING WANG , XINLI EMILY WU
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
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公开(公告)号：US20220251587A1
公开(公告)日：2022-08-11
申请号：US17717357
申请日：2022-04-11
发明人： STEPHEN LAWRENCE GASIOR , WILLIAM JAMES GORDON-KAMM , KEITH S LOWE , DAVID J PETERSON , SERGEI SVITASHEV , JOSHUA K YOUNG
IPC分类号： C12N15/82
摘要： Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.
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公开(公告)号：US20200270622A1
公开(公告)日：2020-08-27
申请号：US16650334
申请日：2018-09-19
发明人： ALEXANDRE DA SILVA CONCEICAO , ALLISON M FINCH , WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE
IPC分类号： C12N15/82
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include tissue-preferred promoters operably linked to morphogenic genes. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein operably linked to a morphogenic gene. DNA constructs comprising a promoter operably linked to a morphogenic gene and further comprising a heterologous nucleotide sequence of interest are also provided.
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公开(公告)号：US20200263189A1
公开(公告)日：2020-08-20
申请号：US16755432
申请日：2018-10-12
发明人： TIM FOX , WILLIAM JAMES GORDON-KAMM , RACHEL CAROL HUEGEL , KEITH S LOWE , JON AARON TUCKER REINDERS , HUAXUN YE
摘要： Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.
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公开(公告)号：US20230091338A1
公开(公告)日：2023-03-23
申请号：US17904722
申请日：2021-02-22
发明人： AJITH ANAND , MAREN L ARLING , PIERLUIGI BARONE , HYEON-JE CHO , WILLIAM JAMES GORDON-KAMM , SANDEEP KUMAR , BRIAN LENDERTS , KEITH S LOWE , SERGEI SVITASHEV , XINLI EMILY WU
摘要： Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.
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公开(公告)号：US20220154203A1
公开(公告)日：2022-05-19
申请号：US17437791
申请日：2020-03-10
IPC分类号： C12N15/82 , C07K14/415
摘要： The methods disclosed herein provide for clonal plant production using a morphogenic gene to produce non-transgenic and transgenic plants wherein the morphogenic gene does not integrate into the genome of the plant.
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