公开(公告)号：US20170335337A1

公开(公告)日：2017-11-23

申请号：US15660390

申请日：2017-07-26

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E I DU PONT DE NEMOURS AND COMPANY

发明人： SCOTT DIEHN ,  AJIT NOTT ,  DAVID A. SELINGER ,  CARL SIMMONS ,  PRIYANKA BHYRI ,  VENKATA S. TAVVA

IPC分类号： C12N15/82 ,  C12Q1/68 ,  G01N33/573

CPC分类号： C12N15/8216

摘要： The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.

公开(公告)号：US20190316142A1

公开(公告)日：2019-10-17

申请号：US16429846

申请日：2019-06-03

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E. I. DU PONT DE NEMOURS AND COMPANY

发明人： SCOTT DIEHN ,  AJIT NOTT ,  DAVID A SELINGER ,  CARL SIMMONS ,  PRIYANKA BHYRI ,  VENKATA S TAVVA

IPC分类号： C12N15/82

摘要： The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.

公开(公告)号：US20150184177A1

公开(公告)日：2015-07-02

申请号：US14660076

申请日：2015-03-17

申请人： E I DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL INC

发明人： SCOTT DIEHN ,  AJIT NOTT ,  DAVID A SELINGER ,  CARL SIMMONS ,  PRIYANKA BHYRI ,  VENKATA S TAVVA

IPC分类号： C12N15/82 ,  G01N33/573 ,  C12Q1/68

CPC分类号： C12N15/8216

摘要： The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.

摘要翻译： 本发明涉及基因表达调控序列，特别是作为基因表达增强子的内含子，与这些内含子内源性相关的启动子和终止子序列。 这些内含子增强子序列在启动子序列附近的存在导致基因表达的增强。 还描述了发现这种新的内含子增强子序列并将其用于产生转基因植物的方法。

公开(公告)号：US20220119830A1

公开(公告)日：2022-04-21

申请号：US17645631

申请日：2021-12-22

申请人： E. I. DU PONT DE NEMOURS AND COMPANY ,  PIONEER HI-BRED INTERNATIONAL, INC.

发明人： SCOTT DIEHN ,  AJIT NOTT ,  DAVID SELINGER ,  CARL SIMMONS ,  PRIYANKA BHYRI ,  VENKATA S TAVVA

IPC分类号： C12N15/82

摘要： The invention relates to gene expression regulatory sequences, specifically introns that act as enhancers of gene expression, the promoter and terminator sequences endogenously associated with these introns. Presence of these intronic enhancer sequences in proximity to promoter sequences leads to enhancement of gene expression. Methods of finding such new intronic enhancer sequences and using them to generate transgenic plants are also described.

公开(公告)号：US20230348928A1

公开(公告)日：2023-11-02

申请号：US18006423

申请日：2021-08-04

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： PRIYANKA BHYRI ,  NANDINI DASGUPTA ,  KRUPA DESHMUKH ,  SCOTT DIEHN ,  KNUT MEYER ,  GILDA RAUSCHER ,  KEVIN G. RIPP ,  LYNNE EILEEN SIMS

IPC分类号： C12N15/82

CPC分类号： C12N15/8279 ,  C12N15/8274 ,  C12N15/8262

摘要： The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.

公开(公告)号：US20210388369A1

公开(公告)日：2021-12-16

申请号：US17288509

申请日：2019-11-05

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： PRIYANKA BHYRI ,  AMITABH MOHANTY ,  PHANIKANTH TURLAPATI

IPC分类号： C12N15/82 ,  C07K14/415

摘要： The disclosure relates to gene expression modulation elements from plants and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the expression modulating elements, and methods using the same.