公开(公告)号：US20170196950A1

公开(公告)日：2017-07-13

申请号：US15316372

申请日：2015-06-05

申请人： PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

发明人： Carolina LLANOS MUNOZ ,  Alexis Mikes KALERGIS PARRA ,  Fabian Alejandro VEGA TAPIA ,  Andy lgor TORRES BAEZA

IPC分类号： A61K39/00 ,  G01N33/569 ,  G01N33/68 ,  C12N5/0784

CPC分类号： A61K39/0008 ,  A61K35/15 ,  A61K2039/5154 ,  A61K2039/577 ,  C12N5/064 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2501/04 ,  C12N2501/22 ,  C12N2501/2304 ,  C12N2501/999 ,  C12N2502/1114 ,  C12N2502/1192 ,  G01N33/56972 ,  G01N33/6869 ,  G01N2333/5412 ,  G01N2333/5434 ,  G01N2333/70503 ,  G01N2333/70532 ,  G01N2333/70539 ,  G01N2333/70578

摘要： The invention relates to a method for producing tolerogenic dendritic cells (tolDCs) with specific antigens, comprising the steps of: (a) culturing precursors of dendritic cells in an animal-serum-free medium, using cytokines, IL-4 and GM-CSF, in order to differentiate same in dendritic cells; (b) producing apoptotic cells; (c) culturing the dendritic cells obtained in step (b) in the presence of compounds having anti-inflammatory activity; (d) co-culturing the dendritic cells from step (d) with the apoptotic cells from step (c), such as to stimulate the endocytosis of the apoptotic cells by the dendritic cells; (e) and, by means of identification based on phenotypic evaluation, determining the production of tolerogenic dendritic cells (tolDCs) with specific antigens. The invention also relates to the tolDC cells produced with said method and to the use of said tolDCs with specific antigens in the production of a drug suitable for the treatment of systemic lupus erythematosus.