公开(公告)号：US20200275829A1

公开(公告)日：2020-09-03

申请号：US16289555

申请日：2019-02-28

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION ,  THE ASAN FOUNDATION ,  UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION ,  OSONG MEDICAL INNOVATION FOUNDATION

Inventor： Seong Han KIM ,  Seunghun LEE ,  Hoon Cheol JANG ,  Bumju KIM ,  Viet Hoan LE ,  Wonyeong PARK ,  Ki Hean KIM ,  Seung Jae MYUNG ,  Seungrag LEE

IPC: A61B1/04 ,  A61B5/00 ,  A61K31/496

Abstract: A fluorescence endoscopic method for visualization of colorectal tissue using a fluoroquinolone antibiotic and a method for diagnosis of lesions of colorectal tissue using the same. The fluorescence endoscopic method for visualization of colorectal tissue includes staining goblet cells of the colorectal tissue with moxifloxacin, which is a fluoroquinolone antibiotic, and exciting the stained goblet cells with single photons in the near UV region or in the visible region, followed by fluorescence endoscopic photographing of the goblet cells, thereby enabling acquisition of morphological information on living tissue without damage to or destruction of the colorectal tissue, while allowing diagnosis of lesions of the colon based on the morphological information on living tissue such as information on the distribution of the goblet cells. Specifically, the fluorescence endoscopic method includes: a colorectal tissue staining step in which the crypt of the colorectal tissue is stained with a fluoroquinolone antibiotic; an image pickup unit insertion step in which an image pickup unit is inserted into the colon; a light irradiation step in which the colorectal tissue is irradiated with excitation light from a light source of the image pickup unit; a colorectal tissue photographing step in which the colorectal tissue is photographed using a photographing device of the image pickup unit through the fluoroquinolone antibiotic fluorescence-excited by light in the light irradiation step, wherein, in the colorectal tissue staining step, goblet cells of the crypt are stained with the fluoroquinolone antibiotic; in the light irradiation step, the light source emits single photons as excitation light inducing fluorescence of the fluoroquinolone antibiotic, the excitation light from the light source having a wavelength band within the near UV and visible regions; and, in the image pickup unit insertion step, the image pickup unit is a general fluorescence endoscope or a confocal endoscopic microscope.

公开(公告)号：US20160202263A1

公开(公告)日：2016-07-14

申请号：US14745973

申请日：2015-06-22

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Hyunsoo MOON ,  Dokyoung KIM ,  Ki Hean KIM ,  Hye Gun RYU

IPC: G01N33/58 ,  G01N21/64 ,  C07D311/92 ,  G01N33/68 ,  C07D311/78 ,  C07D311/94

CPC classification number: G01N33/582 ,  A61K49/0021 ,  C07D311/78 ,  C07D311/92 ,  C07D311/94 ,  G01N21/6428 ,  G01N33/6896 ,  G01N2021/6439 ,  G01N2333/4709 ,  G01N2800/2821

Abstract: Provided are acedan derivatives having an extended π bond, a method for preparing the acedan derivatives, and a method for two-photon microscopy imaging of amyloid-beta plaque using the acedan derivatives; more particularly, to two-photon absorbing fluorescent compounds having a longer absorption wavelength and emission wavelength than acedan and acedan derivatives which are conventional two-photon absorbing fluorophores. The compounds provided may be usefully used for in vivo imaging studies by imaging cells or tissue using the compounds, and may also be usefully used for diagnosing Alzheimer's disease by imaging amyloid-beta plaque using the compounds.

Abstract translation: 提供的是具有延伸＆ 键，一种制备acedan衍生物的方法，以及使用acedan衍生物的淀粉样蛋白-β斑块双光子显微镜成像方法。 更具体地说，涉及具有比作为常规双光子吸收荧光团的常规阿敏和acedan衍生物更长的吸收波长和发射波长的双光子吸收荧光化合物。 所提供的化合物可以有用地用于通过使用化合物成像细胞或组织的体内成像研究，并且还可以有用地用于通过使用该化合物成像淀粉样蛋白-β斑块来诊断阿尔茨海默氏病。

公开(公告)号：US20200278299A1

公开(公告)日：2020-09-03

申请号：US16289536

申请日：2019-02-28

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Seonghan KIM ,  Ki Hean KIM ,  Myoung Joon KIM ,  Seunghun LEE ,  Hoon Cheol JANG ,  Viet Hoan LE ,  Soo Hyun PARK

IPC: G01N21/64 ,  G01N33/50 ,  G01N33/58

Abstract: A method for visualization of conjunctival cells using fluoroquinolone antibiotics and a method for diagnosis of ocular lesions using the same. The method for visualization of conjunctival cells using fluoroquinolone antibiotics includes staining goblet cells of ocular conjunctiva with moxifloxacin, which is a fluoroquinolone antibiotic, and exciting the stained goblet cells with single photons in the near-UV region or in the visible region, followed by fluorescence photographing of the goblet cells, thereby enabling acquisition of morphological information on living tissue without damage to or destruction of the ocular conjunctiva. Specifically, the method for visualization of conjunctival cells includes: a conjunctiva staining step in which ocular conjunctiva is stained with a fluoroquinolone antibiotic; a light irradiation step in which the ocular conjunctiva stained with the fluoroquinolone antibiotic is irradiated with light from a light source; and a conjunctiva photographing step in which the ocular conjunctiva is photographed using an image pickup unit through the fluoroquinolone antibiotic fluorescence-excited by light in the light irradiation step, wherein, in the conjunctiva staining step, goblet cells of the ocular conjunctiva are stained with the fluoroquinolone antibiotic; in the light irradiation step, the light source emits single photons; and, in the conjunctiva photographing step, the image pickup unit photographing the ocular conjunctiva is a high-magnification fluorescence microscope or a slit lamp microscope.

公开(公告)号：US20160116455A1

公开(公告)日：2016-04-28

申请号：US14888883

申请日：2014-03-10

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han AHN ,  Dokyoung KIM ,  Sungjee KIM ,  Ki Hean KIM

IPC: G01N33/50 ,  C07C223/06

CPC classification number: G01N33/5023 ,  C07C223/06 ,  C09K11/06 ,  C12Q1/485 ,  G01N33/574 ,  G01N33/582 ,  G01N2333/91205 ,  G01N2333/9121 ,  G01N2500/10

Abstract: A fluorescent probe for detecting a tyrosine kinase using a compound having an ortho-hydroxy-benzaldehyde structure, and use thereof are provided. The fluorescent probe can show a change in fluorescence when the compound binds with a tyrosine kinase. The compound can be readily synthesized and has high stability and low cytotoxicity in vivo. The fluorescent probe can be used to image cells or tissues overexpressing the tyrosine kinase, the fluorescent probe can be effectively used in a composition for imaging the tissues and a method of imaging the tissues. Also, the fluorescent probe can be used to image cancer cells or tissues since the fluorescent probe can exhibit fluorescence when the fluorescent probe binds to the cancer cells or tissues overexpressing the tyrosine kinase.

Abstract translation: 提供了使用具有邻羟基 - 苯甲醛结构的化合物检测酪氨酸激酶的荧光探针及其用途。 当化合物与酪氨酸激酶结合时，荧光探针可以显示荧光的变化。 该化合物可以容易地合成并且在体内具有高稳定性和低细胞毒性。 荧光探针可用于对过量表达酪氨酸激酶的细胞或组织进行成像，荧光探针可以有效地用于组织中用于成像组织和使组织成像的方法。 此外，荧光探针可以用于对癌细胞或组织进行成像，因为当荧光探针结合到癌细胞或过表达酪氨酸激酶的组织时，荧光探针可以显示出荧光。