公开(公告)号：US20130334120A1

公开(公告)日：2013-12-19

申请号：US13918193

申请日：2013-06-14

Applicant: President And Fellows Of Harvard College

Inventor： Donald E. Ingber ,  Joo Hun Kang ,  Richard C. Terry ,  Michael Super ,  Ryan M. Cooper ,  Karel Domansky ,  Chong Wing Yung

IPC: B03C1/28

CPC classification number: B03C1/28 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26

Abstract: A system and method for removing a target species from a fluid source is provided. The system includes a reciprocating fluid cleansing device, including a processing chamber with a port at a first end for fluid passage and a movable plunger at a second end, wherein the plunger in contact with a fluid includes a motorized mixing element for mixing the fluid with species-targeting magnetic particles. Motion of the plunger in a first direction transfers a first volume of the fluid from the fluid source into the processing chamber. Motion of the plunger in a second direction transfers the first volume of the fluid from the processing chamber to a fluid destination. At least one magnetic element provides a magnetic field gradient within the processing chamber. A connector connects the port of the first processing chamber to the fluid source and the fluid destination.

Abstract translation: 提供了用于从流体源中去除目标物种的系统和方法。 该系统包括往复式流体清洁装置，其包括处理室，其具有用于流体通道的第一端的端口和在第二端处的可移动柱塞，其中与流体接触的柱塞包括用于将流体与 以物种为目标的磁性颗粒。 柱塞在第一方向上的运动将第一体积的流体从流体源传送到处理室。 柱塞在第二方向的运动将第一体积的流体从处理室传送到流体目的地。 至少一个磁性元件在处理室内提供磁场梯度。 连接器将第一处理室的端口连接到流体源和流体目的地。

公开(公告)号：US11059050B2

公开(公告)日：2021-07-13

申请号：US16414168

申请日：2019-05-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Joo Hun Kang ,  Donald E. Ingber ,  Michael Super

IPC: B03C1/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28 ,  C12N1/20 ,  B03C1/025

Abstract: Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).

公开(公告)号：US10357780B2

公开(公告)日：2019-07-23

申请号：US15522686

申请日：2015-10-27

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Joo Hun Kang ,  Donald E. Ingber ,  Michael Super

IPC: B03C1/00 ,  C12N1/20 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28 ,  B03C1/025

Abstract: Disclosed herein is an improved method for magnetic capture of target molecules (e.g., microbes) in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation and/or detection of microbes and microbe-associated molecular pattern (MAMP) (including, e.g., but not limited to, a cell component of microbes, lipopolysaccharides (LPS), and/or endotoxin).