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公开(公告)号：US11859212B2
公开(公告)日：2024-01-02
申请号：US16611359
申请日：2018-05-02
发明人： Tamihide Matsunaga , Takahiro Iwao , Daichi Onozato , Isamu Ogawa
CPC分类号： C12N5/0679 , A61L27/3834 , A61L27/3882 , A61L27/3895 , G01N33/5082 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/998 , C12N2501/999 , C12N2503/04 , C12N2506/45 , C12N2513/00 , C12N2533/70
摘要： An object of the present invention is to prepare a functional intestinal organoid from pluripotent stem cells. An intestinal organoid is prepared from pluripotent stem cells, by the following steps (1) to (4): (1) differentiating pluripotent stem cells into endoderm-like cells; (2) differentiating the endoderm-like cells obtained in step (1) into intestinal stem cell-like cells; (3) culturing the intestinal stem cell-like cells obtained in step (2) to form spheroids; and (4) differentiating the spheroids formed in step (3) to form an intestinal organoid, the step including culture in the presence of a MEK1/2 inhibitor, a DNA methylation inhibitor, a TGF-β receptor inhibitor, and a γ-secretase inhibitor, in addition to an epidermal growth factor, a BMP inhibitor, and a Wnt signal activator.
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公开(公告)号：US20200157507A1
公开(公告)日：2020-05-21
申请号：US16611359
申请日：2018-05-02
发明人： Tamihide Matsunaga , Takahiro Iwao , Daichi Onozato , Isamu Ogawa
摘要： An object of the present invention is to prepare a functional intestinal organoid from pluripotent stem cells. An intestinal organoid is prepared from pluripotent stem cells, by the following steps (1) to (4): (1) differentiating pluripotent stem cells into endoderm-like cells; (2) differentiating the endoderm-like cells obtained in step (1) into intestinal stem cell-like cells; (3) culturing the intestinal stem cell-like cells obtained in step (2) to form spheroids; and (4) differentiating the spheroids formed in step (3) to form an intestinal organoid, the step including culture in the presence of a MEK1/2 inhibitor, a DNA methylation inhibitor, a TGF-β receptor inhibitor, and a γ-secretase inhibitor, in addition to an epidermal growth factor, a BMP inhibitor, and a Wnt signal activator.
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